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Open AccessArticle
A New Approach to Examine Cell–Antibody Avidity with Surface Plasmon Resonance Imaging
by
Richard B. M. Schasfoort
Richard B. M. Schasfoort
Richard B.M. Schasfoort (1959) works at the University of Twente and Vysens B.V., the Netherlands. a [...]
Richard B.M. Schasfoort (1959) works at the University of Twente and Vysens B.V., the Netherlands. He graduated in 1984 as a biotechnology major from the University of Groningen. He defended his PhD thesis for a new approach to biosensor operation in 1989. In 2002, Richard started the biochip group at the University of Twente after receiving the VIDI grant from NWO. He is the co-author of > 100 peer-reviewed papers and editor of the two Handbooks of Surface Plasmon Resonance (2008 and 2017). He is currently developing a new SPR imager in collaboration with the start-up company Vysens B.V. Via thorough market evaluation, the focus of the project is on the development of the CellVysion instrument to measure the avidity of cell–antibody interactions using IP-protected fluidics. It is expected that the CellVysion instrument will be launched in the autumn of 2025.
,
Elise van Doorn
Elise van Doorn
Jos van Weperen
Jos van Weperen
Anouk Mentink
Anouk Mentink
Ruchi Bansal
Ruchi Bansal
1
Department of Bioengineering Technologies, Technical Medical Centre, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
2
Vysens B.V. Fl. Hazemeijerstraat 800/A04, 7555 RJ Hengelo, The Netherlands
*
Author to whom correspondence should be addressed.
Biosensors 2025, 15(9), 559; https://doi.org/10.3390/bios15090559 (registering DOI)
Submission received: 27 June 2025
/
Revised: 25 July 2025
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Accepted: 21 August 2025
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Published: 25 August 2025
Abstract
In recent years, avidity has emerged as a critical parameter in antibody design, yet most current analytical instruments are limited to measuring affinity alone. This study aims to evaluate the capabilities and advantages of a novel surface plasmon resonance imaging instrument, CellVysion, designed to quantify cell–antibody avidity using a continuous antibody density gradient. A key feature of this approach is the identification of a “tipping point”—the specific ligand density, measured in µRIUs, at which cells remain bound to the sensor surface under defined shear flow conditions. In this paper, we present the technical principles and application of this method, demonstrating how avidity can be quantitatively assessed across different antibody–cell line combinations.
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MDPI and ACS Style
Schasfoort, R.B.M.; van Doorn, E.; van Weperen, J.; Mentink, A.; Bansal, R.
A New Approach to Examine Cell–Antibody Avidity with Surface Plasmon Resonance Imaging. Biosensors 2025, 15, 559.
https://doi.org/10.3390/bios15090559
AMA Style
Schasfoort RBM, van Doorn E, van Weperen J, Mentink A, Bansal R.
A New Approach to Examine Cell–Antibody Avidity with Surface Plasmon Resonance Imaging. Biosensors. 2025; 15(9):559.
https://doi.org/10.3390/bios15090559
Chicago/Turabian Style
Schasfoort, Richard B. M., Elise van Doorn, Jos van Weperen, Anouk Mentink, and Ruchi Bansal.
2025. "A New Approach to Examine Cell–Antibody Avidity with Surface Plasmon Resonance Imaging" Biosensors 15, no. 9: 559.
https://doi.org/10.3390/bios15090559
APA Style
Schasfoort, R. B. M., van Doorn, E., van Weperen, J., Mentink, A., & Bansal, R.
(2025). A New Approach to Examine Cell–Antibody Avidity with Surface Plasmon Resonance Imaging. Biosensors, 15(9), 559.
https://doi.org/10.3390/bios15090559
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