Immunosensor Enhanced with Silver Nanocrystals for On-Chip Prostate-Specific Antigen Detection
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals and Equipment
2.2. Synthesis of Silver Nanocrystals
2.3. Immunosensor Fabrication Process
2.4. Experimental Measurements
3. Results and Discussion
3.1. Characterization of Nanomaterials
3.2. Analytical Application of the Immunosensor
3.2.1. Stability, Reproducibility, and Selectivity
3.2.2. Application in the Detection of PSA
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Immunosensor/Electrode Material | Detection Method | Linear Range (ng/mL) | LOD (ng/mL) | Ref. |
---|---|---|---|---|
BSA/Anti-PSA/PTH/GQD/MG /SPE | DPV | 0.0125–1.0, 1.0–80.0 | 0.005 | [31] |
AuNPs-poly(FFP-AM)-RGO/AuE | DPV | 0.01–110 | 0.001 | [32] |
SPE/AuNP//Anti-body/BSA | DPV | 1.0–8.0 | 0.55 | [33] |
3D-GR-Aua/GCE | CV, DPV | 0–10 | 0.59 | [34] |
GCE/AgNP/Ab/BSA | CV | 2.5–11 | 1.7 × 10−1 | [18] |
(MoS2@Cu2O)-Au nanocomposite | L-SAW | 0.2–5 | 0.076 | [35] |
MWCNTs | EIS | 0–500 | 1.18 | [36] |
Pipette-tip type biosensor | Fluorescence | 0–10 | 1.2 | [37] |
GCE/AgNC/EDC-NHS/Ab/BSA | SWV | 1–10 | 1.14 | This work |
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Okhai, T.A.; Mokwebo, K.V.; Oranzie, M.; Feleni, U.; Snyman, L.W. Immunosensor Enhanced with Silver Nanocrystals for On-Chip Prostate-Specific Antigen Detection. Biosensors 2025, 15, 428. https://doi.org/10.3390/bios15070428
Okhai TA, Mokwebo KV, Oranzie M, Feleni U, Snyman LW. Immunosensor Enhanced with Silver Nanocrystals for On-Chip Prostate-Specific Antigen Detection. Biosensors. 2025; 15(7):428. https://doi.org/10.3390/bios15070428
Chicago/Turabian StyleOkhai, Timothy A., Kefilwe V. Mokwebo, Marlon Oranzie, Usisipho Feleni, and Lukas W. Snyman. 2025. "Immunosensor Enhanced with Silver Nanocrystals for On-Chip Prostate-Specific Antigen Detection" Biosensors 15, no. 7: 428. https://doi.org/10.3390/bios15070428
APA StyleOkhai, T. A., Mokwebo, K. V., Oranzie, M., Feleni, U., & Snyman, L. W. (2025). Immunosensor Enhanced with Silver Nanocrystals for On-Chip Prostate-Specific Antigen Detection. Biosensors, 15(7), 428. https://doi.org/10.3390/bios15070428