Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture Surface Preparation
2.2. Vagal Afferent Neuron Dissection and Culture
2.3. Effector Molecules
2.4. Intracellular Calcium Flux Measurements
2.5. Immunocytochemistry and Imaging
2.6. In-House Fabricated Microelectrode Arrays
2.7. High-Density Microelectrode Arrays
2.8. Statistical Analysis
3. Results and Discussion
3.1. Influence of Culture Conditions on VAN Morphology
3.2. Live-Cell Calcium Imaging
3.3. Extracellular Recordings of VANs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Girardi, G.; Zumpano, D.; Goshi, N.; Raybould, H.; Seker, E. Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules. Biosensors 2023, 13, 601. https://doi.org/10.3390/bios13060601
Girardi G, Zumpano D, Goshi N, Raybould H, Seker E. Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules. Biosensors. 2023; 13(6):601. https://doi.org/10.3390/bios13060601
Chicago/Turabian StyleGirardi, Gregory, Danielle Zumpano, Noah Goshi, Helen Raybould, and Erkin Seker. 2023. "Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules" Biosensors 13, no. 6: 601. https://doi.org/10.3390/bios13060601