Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer
Abstract
:1. Introduction
2. Establishment of Biosensing System
2.1. Both the Excitation Wavelength and Incidence Angle Were Optimized
2.2. System Setup
3. Results and Discussion
3.1. Dynamic and RIR
3.2. SPRi Detection of Apoptosis in Cancer Cells with a Caspase-3 Inhibitor
3.2.1. Materials and Reagents
Chemicals and Materials
Cell Culture and Reagents
Apoptosis Assay by Annexin V/PI Staining and Flow Cytometry
Western Blotting
3.2.2. ISPRi Detection of Cleaved Caspase-3 (C-CASP-3) with Its Inhibitor Z-DEVD-FMK
3.2.3. Detection of Activated Caspase-3 with Its Inhibitor Z-DEVD-FMK by ISPRi Biosensor
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yuan, X.; Niu, Z.; Liu, L.; Zeng, Y.; Ma, L.; Nie, Z.; Tian, Z.; Kai, D.; Zhang, F.; Liu, G.; et al. Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer. Biosensors 2023, 13, 946. https://doi.org/10.3390/bios13100946
Yuan X, Niu Z, Liu L, Zeng Y, Ma L, Nie Z, Tian Z, Kai D, Zhang F, Liu G, et al. Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer. Biosensors. 2023; 13(10):946. https://doi.org/10.3390/bios13100946
Chicago/Turabian StyleYuan, Xin, Zhenxiao Niu, Lang Liu, Youjun Zeng, Lin Ma, Zhaogang Nie, Zhen Tian, Dongyun Kai, Fangteng Zhang, Guanyu Liu, and et al. 2023. "Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer" Biosensors 13, no. 10: 946. https://doi.org/10.3390/bios13100946