An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Preparation of RNA from Cell Line and Ovarian Cancer Samples
2.3. Magnetic Isolation and Purification of HOTAIR lncRNA Target
2.4. Electrochemical Detection of HOTAIR lncRNAs
2.5. Quantitative Reverse-Transcription Polymerase Chain Reaction (RT-qPCR)
3. Results and Discussion
3.1. Assay Principle
3.2. Analytical Performance of the Assay
3.3. LncRNA Detection in Ovarian Cancer Patient Samples
3.4. Advantages and Disadvantages of the Assay
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oligonucleotides | 5′-Sequences-3′ |
---|---|
HOTAIR capture probe (Biotinylated) | ATC AAT TAA TTA GCG CCT CCC AGT CCC/3Bio |
Synthetic HOTAIR lncRNA target | GGG ACU GGG AGG CGC UAA UUA AUU GAU UCC UUU GGA CUG UAA AAU AUG GCG GCG U |
miR-891 | UGC AAC GAA CCU GAG CCA CUG A |
miR-486 | UCC UGU ACU GAG CUG CCC CGA G |
HOTAIR Forward primer sequence | AAG CAC CTC TAT CTC AGC CG |
HOTAIR Reverse primer sequence | AGA ACC CTC TGA CAT TTG CCT |
GAPDH Forward primer sequence | CCG GGA AAC TGT GGC GTG ATG G |
GAPDH Reverse primer sequence | AGG TGG AGG AGT GGG TGT CGC TGT T |
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Clack, K.; Soda, N.; Kasetsirikul, S.; Kline, R.; Salomon, C.; Shiddiky, M.J.A. An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples. Biosensors 2022, 12, 287. https://doi.org/10.3390/bios12050287
Clack K, Soda N, Kasetsirikul S, Kline R, Salomon C, Shiddiky MJA. An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples. Biosensors. 2022; 12(5):287. https://doi.org/10.3390/bios12050287
Chicago/Turabian StyleClack, Kimberley, Narshone Soda, Surasak Kasetsirikul, Richard Kline, Carlos Salomon, and Muhammad J. A. Shiddiky. 2022. "An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples" Biosensors 12, no. 5: 287. https://doi.org/10.3390/bios12050287