A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Bacterial Strains and Culture Conditions
2.3. Expression, Purification, and Characterization of Lactate Utilization Operon Regulator LldR
2.4. Fluorescence-Based Thermal Shift (FTS) Assay
2.5. Construction and Purification of Fluorescent l-Lactate Biosensor FILLac
2.6. Characterization of FILLac In-Virto
2.7. Batch Fermentation for Lactate Production
2.8. Jiaosu and Yogurt Samples Preparation
2.9. Quantification of l-Lactate by HPLC, SBA-40D Bioanalyzer and FILLac10N0C
3. Results and Discussion
3.1. STLldR as a Specific Recognition Element for l-Lactate
3.2. Design and Optimization of the Fluorescent l-Lactate Biosensor
3.3. Characterization of the Optimal l-Lactate Biosensor FILLac10N0C
3.4. Performance of FILLac10N0C in l-Lactate Quantitation
3.5. Quantitation of l-Lactate in Different Fermentation Samples by FILLac10N0C
3.6. Determination of l-Lactate in Food Samples by FILLac10N0C
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xu, X.; Xu, R.; Hou, S.; Kang, Z.; Lü, C.; Wang, Q.; Zhang, W.; Wang, X.; Xu, P.; Gao, C.; et al. A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer. Biosensors 2022, 12, 1111. https://doi.org/10.3390/bios12121111
Xu X, Xu R, Hou S, Kang Z, Lü C, Wang Q, Zhang W, Wang X, Xu P, Gao C, et al. A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer. Biosensors. 2022; 12(12):1111. https://doi.org/10.3390/bios12121111
Chicago/Turabian StyleXu, Xianzhi, Rong Xu, Shuang Hou, Zhaoqi Kang, Chuanjuan Lü, Qian Wang, Wen Zhang, Xia Wang, Ping Xu, Chao Gao, and et al. 2022. "A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer" Biosensors 12, no. 12: 1111. https://doi.org/10.3390/bios12121111