Nonbiodegradable Spiegelmer-Driven Colorimetric Biosensor for Bisphenol A Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Determination of Dissociation Constants
2.3. Biostability Assays
2.4. Direct Competitive Enzyme-Lined Aptamer Assays (ELAAs) in Which BLI Is Utilized
2.5. Synthesis of AuNPs
2.6. AuNP-Based Colorimetric Detection of BPA
3. Results and Discussion
3.1. Design and Characterization of the Spiegelmer
3.2. Biostability of the Spiegelmer
3.3. Direct Competitive to Detect BPA and Cross-Reactivity
3.4. AuNP-Based Colorimetric Detection
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Categories | Detection Methods | LOD | Dynamic Range | Ref. |
---|---|---|---|---|
Carbon nanotube transistor sensor | SwCNT-FET capacitance based sensor | 1 pM | - | [30] |
Capacitance sensor | AAO-based capacitance sensor | 1 nM | 1 nm~100 nm | [31] |
Colorimetric sensor | GNA and aptamer | 0.1 ng/mL | 0.1~100 ng/mL | [24] |
Colorimetric sensor | GNA and aptamer | 1 pg/mL | 1 pg/mL~1 mg/mL | [32] |
Fluorescent sensor | Small GNP’s with functionalized aptamer | 0.1 ng/mL | 1~10,000 ng/mL | [33] |
Electrochemical sensors | Competitive cDNA aptasensors | 0.284 pg/mL | 0.284~1000 pg/mL | [34] |
Electrochemical sensor | DPV using AuNP/MWCNT/GCE | 4 nM | 0.01~0.7 µM | [35] |
Electrochemical sensor | DPV using AuNP–rGO–MWCNTs/GCE | 1 nM | 5 nM~20 µM | [36] |
This method | GNP with L-form DNA aptamer | 0.1 ng/mL | 0.1 ng/mL~1 mg/mL |
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Ren, S.; Cho, S.; Lin, R.; Gedi, V.; Park, S.; Ahn, C.W.; Lee, D.-K.; Lee, M.-H.; Lee, S.; Kim, S. Nonbiodegradable Spiegelmer-Driven Colorimetric Biosensor for Bisphenol A Detection. Biosensors 2022, 12, 864. https://doi.org/10.3390/bios12100864
Ren S, Cho S, Lin R, Gedi V, Park S, Ahn CW, Lee D-K, Lee M-H, Lee S, Kim S. Nonbiodegradable Spiegelmer-Driven Colorimetric Biosensor for Bisphenol A Detection. Biosensors. 2022; 12(10):864. https://doi.org/10.3390/bios12100864
Chicago/Turabian StyleRen, Shuo, Samuel Cho, Ruixan Lin, Vinayakumar Gedi, Sunyoung Park, Chul Woo Ahn, Dong-Ki Lee, Min-Ho Lee, Sangwook Lee, and Soyoun Kim. 2022. "Nonbiodegradable Spiegelmer-Driven Colorimetric Biosensor for Bisphenol A Detection" Biosensors 12, no. 10: 864. https://doi.org/10.3390/bios12100864