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A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening

School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia
Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
Genea, Sydney, NSW 2000, Australia
SUStech-UTS Joint Research Centre for Biomedical Materials & Devices, Southern University of Science and Technology, Shenzhen 518055, China
Serology and Virology Division, NSW Health Pathology, Prince of Wales Hospital, Sydney, NSW 2031, Australia
School of Women’s and Children’s Health, University of New South Wales, Sydney, NSW 2052, Australia
Author to whom correspondence should be addressed.
Biosensors 2021, 11(10), 369;
Received: 26 August 2021 / Revised: 19 September 2021 / Accepted: 28 September 2021 / Published: 2 October 2021
The COVID-19 pandemic has changed people’s lives and has brought society to a sudden standstill, with lockdowns and social distancing as the preferred preventative measures. To lift these measurements and reduce society’s burden, developing an easy-to-use, rapid, and portable system to detect SARS-CoV-2 is mandatory. To this end, we developed a portable and semi-automated device for SARS-CoV-2 detection based on reverse transcription loop-mediated isothermal amplification followed by a CRISPR/Cas12a reaction. The device contains a heater element mounted on a printed circuit board, a cooler fan, a proportional integral derivative controller to control the temperature, and designated areas for 0.2 mL Eppendorf® PCR tubes. Our system has a limit of detection of 35 copies of the virus per microliter, which is significant and has the capability of being used in crisis centers, mobile laboratories, remote locations, or airports to diagnose individuals infected with SARS-CoV-2. We believe the current methodology that we have implemented in this article is beneficial for the early screening of infectious diseases, in which fast screening with high accuracy is necessary. View Full-Text
Keywords: SARS-CoV-2; COVID-19; point of care testing; rapid diagnostics SARS-CoV-2; COVID-19; point of care testing; rapid diagnostics
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MDPI and ACS Style

Rezaei, M.; Razavi Bazaz, S.; Morshedi Rad, D.; Shimoni, O.; Jin, D.; Rawlinson, W.; Ebrahimi Warkiani, M. A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening. Biosensors 2021, 11, 369.

AMA Style

Rezaei M, Razavi Bazaz S, Morshedi Rad D, Shimoni O, Jin D, Rawlinson W, Ebrahimi Warkiani M. A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening. Biosensors. 2021; 11(10):369.

Chicago/Turabian Style

Rezaei, Meysam, Sajad Razavi Bazaz, Dorsa Morshedi Rad, Olga Shimoni, Dayong Jin, William Rawlinson, and Majid Ebrahimi Warkiani. 2021. "A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening" Biosensors 11, no. 10: 369.

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