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Article

Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters

by 1,†, 2,†, 1, 1 and 1,*
1
Institute of Pharmaceutical Biotechnology and the Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310012, China
2
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310012, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biosensors 2021, 11(1), 13; https://doi.org/10.3390/bios11010013
Received: 20 November 2020 / Revised: 20 December 2020 / Accepted: 28 December 2020 / Published: 30 December 2020
(This article belongs to the Special Issue Biosensors for Diagnosis and Monitoring)
High levels of blood glucose are always associated with numerous complications including cholesterol abnormalities. Therefore, it is important to simultaneously monitor blood glucose and cholesterol levels in patients with diabetes during the management of chronic diseases. In this study, a glucose dehydrogenase from Aspergillus oryzae TI and a cholesterol oxidase from Chromobacterium sp. DS-1 were displayed on the surface of Saccharomyces cerevisiae, respectively, using the yeast surface display system at a high copy number. In addition, two whole-cell biosensors were constructed through the immobilization of the above yeast cells on electrodes, for electrochemical detection of glucose and cholesterol. The assay time was 8.5 s for the glucose biosensors and 30 s for the cholesterol biosensors. Under optimal conditions, the cholesterol biosensor exhibited a linear range from 2 to 6 mmol·L−1. The glucose biosensor responded efficiently to the presence of glucose at a concentration range of 20–600 mg·dL−1 (1.4–33.3 mmol·L−1) and showed excellent anti-xylose interference properties. Both biosensors exhibited good performance at room temperature and remained stable over a three-week storage period. View Full-Text
Keywords: whole-cell biosensor; yeast surface display; cholesterol oxidase; glucose dehydrogenase; electrochemical detection whole-cell biosensor; yeast surface display; cholesterol oxidase; glucose dehydrogenase; electrochemical detection
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MDPI and ACS Style

Zhao, S.; Guo, D.; Zhu, Q.; Dou, W.; Guan, W. Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters. Biosensors 2021, 11, 13. https://doi.org/10.3390/bios11010013

AMA Style

Zhao S, Guo D, Zhu Q, Dou W, Guan W. Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters. Biosensors. 2021; 11(1):13. https://doi.org/10.3390/bios11010013

Chicago/Turabian Style

Zhao, Shiyao, Dong Guo, Quanchao Zhu, Weiwang Dou, and Wenjun Guan. 2021. "Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters" Biosensors 11, no. 1: 13. https://doi.org/10.3390/bios11010013

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