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Open AccessArticle

The Effect of Optically Induced Dielectrophoresis (ODEP)-Based Cell Manipulation in a Microfluidic System on the Properties of Biological Cells

by Po-Yu Chu 1,†, Chia-Hsun Hsieh 2,3,4,†, Chien-Ru Lin 5 and Min-Hsien Wu 3,5,6,*
1
Ph.D. Program in Biomedical Engineering, Chang Gung University, Taoyuan City 33302, Taiwan
2
Division of Haematology/Oncology, Department of Internal Medicine, New Taipei Municipal Hospital, New Taipei City 23600, Taiwan
3
Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33302, Taiwan
4
College of Medicine, Chang Gung University, Taoyuan City 33302, Taiwan
5
Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan City 33302, Taiwan
6
Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan
*
Author to whom correspondence should be addressed.
Chu and Hsieh contributed equally to this work.
Biosensors 2020, 10(6), 65; https://doi.org/10.3390/bios10060065
Received: 11 May 2020 / Revised: 3 June 2020 / Accepted: 14 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Microfluidic Bio-Sensors and Their Applications)
Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed—Whether, the ODEP field affects the native properties of cells. To address this issue, we explored the effect of ODEP electrical conditions on cellular properties. Within the experimental conditions tested, the ODEP-based cell manipulation with the largest velocity occurred at 10 Vpp and 1 MHz, for the two cancer cell types explored. Under this operating condition, however, the cell viability of cancer cells was significantly affected (e.g., 70.5 ± 10.0% and 50.6 ± 9.2% reduction for the PC-3 and SK-BR-3 cancer cells, respectively). Conversely, the exposure of cancer cells to the ODEP electrical conditions of 7–10 Vpp and 3–5 MHz did not significantly alter the cell viability, cell metabolic activity, and the EpCAM, VIM, and ABCC1 gene expression of cancer cells. Overall, this study fundamentally investigated the effect of ODEP electrical conditions on the cellular properties of cancer cells. The information obtained is crucially important for the utilization of ODEP-based cell manipulation in a microscale system for various applications. View Full-Text
Keywords: optically induced dielectrophoresis; microfluidic systems; cell manipulation; cell property; cell physiology optically induced dielectrophoresis; microfluidic systems; cell manipulation; cell property; cell physiology
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Chu, P.-Y.; Hsieh, C.-H.; Lin, C.-R.; Wu, M.-H. The Effect of Optically Induced Dielectrophoresis (ODEP)-Based Cell Manipulation in a Microfluidic System on the Properties of Biological Cells. Biosensors 2020, 10, 65.

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