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Open AccessArticle

Pectobacterium atrosepticum Biosensor for Monitoring Blackleg and Soft Rot Disease of Potato

1
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
2
Institute of Nanotechnology, CNR-Nanotec, Via per Monteroni, 73100 Lecce, Italy
3
CNR-ISPA, Istituto di Scienze delle Produzioni Alimentari-Consiglio Nazionale delle Ricerche, 73100 Lecce, Italy
4
Department of Mathematics and Physics “Ennio De Giorgi”, University of Salento, Omnics Research Group, Via per Monteroni, 73100 Lecce, Italy
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(6), 64; https://doi.org/10.3390/bios10060064
Received: 5 May 2020 / Revised: 6 June 2020 / Accepted: 9 June 2020 / Published: 15 June 2020
(This article belongs to the Section Biosensor and Bioelectronic Devices)
Pectobacterium atrosepticum (Pba) is a quarantine and threatening phytopathogen known as the causal agent of blackleg and soft rot disease of potatoes in many areas. Its early detection is then important to have healthy potato tubers and reduce economic losses. Today, conventional methods such as enzyme-linked immunosorbent-assay (ELISA) and polymerase chain reaction (PCR) are typically used for Pba detection, but they are expensive and time-consuming. Here we report on the optimization of an alternative approach based on an electrochemical impedance immunosensor combining a microfluidic module and a microelectrodes array, and having advantages in terms of low cost, ease of use and portability. For validation and for assessing its performance, the lab-on-chip platform has been compared with two standard methods (ELISA and PCR). View Full-Text
Keywords: lab-on-chip; biosensors; phytopathogen lab-on-chip; biosensors; phytopathogen
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Hashemi Tameh, M.; Primiceri, E.; Chiriacò, M.S.; Poltronieri, P.; Bahar, M.; Maruccio, G. Pectobacterium atrosepticum Biosensor for Monitoring Blackleg and Soft Rot Disease of Potato. Biosensors 2020, 10, 64.

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