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Open AccessArticle

Disturbing-Free Determination of Yeast Concentration in DI Water and in Glucose Using Impedance Biochips

1
Department Back-End of Line, Fraunhofer Institute for Electronic Nano Systems, Technologie-Campus 3, 09126 Chemnitz, Germany
2
Institute for Solid State Physics, University of Jena, 07743 Jena, Germany
3
Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany
4
Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstraße 400, 01328 Dresden, Germany
5
Center for Microtechnologies, Chemnitz University of Technology, Reichenhainer Str. 70, 09126 Chemnitz, Germany
6
Institute for Integrative Nanosciences IFW Dresden, Helmholtzstr. 20, 01069 Dresden, Germany
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(1), 7; https://doi.org/10.3390/bios10010007
Received: 17 December 2019 / Revised: 10 January 2020 / Accepted: 16 January 2020 / Published: 19 January 2020
(This article belongs to the Special Issue Electrochemical (Bio)sensors for Environmental and Food Analyses II)
Deionized water and glucose without yeast and with yeast (Saccharomyces cerevisiae) of optical density OD600 that ranges from 4 to 16 has been put in the ring electrode region of six different types of impedance biochips and impedance has been measured in dependence on the added volume (20, 21, 22, 23, 24, 25 µL). The measured impedance of two out of the six types of biochips is strongly sensitive to the addition of both liquid without yeast and liquid with yeast and modelled impedance reveals a linear relationship between the impedance model parameters and yeast concentration. The presented biochips allow for continuous impedance measurements without interrupting the cultivation of the yeast. A multiparameter fit of the impedance model parameters allows for determining the concentration of yeast (cy) in the range from cy = 3.3 × 107 to cy = 17 × 107 cells/mL. This work shows that independent on the liquid, i.e., DI water or glucose, the impedance model parameters of the two most sensitive types of biochips with liquid without yeast and with liquid with yeast are clearly distinguishable for the two most sensitive types of biochips. View Full-Text
Keywords: biochips; impedance spectroscopy; yeast Saccharomyces cerevisiae; electrical equivalent circuit; biomaterial; biosensing biochips; impedance spectroscopy; yeast Saccharomyces cerevisiae; electrical equivalent circuit; biomaterial; biosensing
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Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S.E.; Schmidt, O.G.; Blaschke, D.; Schmidt, H. Disturbing-Free Determination of Yeast Concentration in DI Water and in Glucose Using Impedance Biochips. Biosensors 2020, 10, 7.

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