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Nanomaterials
Volume 9
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10.3390/nano9091335
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Article

A Label-Free Electrochemical Immunosensor for Detection of the Tumor Marker CA242 Based on Reduced Graphene Oxide-Gold-Palladium Nanocomposite

by
Xin Du
1,
Xiaodi Zheng
1,
Zhenhua Zhang
1,
Xiaofan Wu
2,
Lei Sun
1,
Jun Zhou
1,2 and
Min Liu
1,*
1
Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Institute of Biomedical Sciences, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong, China
2
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials of the Ministry of Education, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin 300071, China
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(9), 1335; https://doi.org/10.3390/nano9091335
Received: 5 August 2019 / Revised: 3 September 2019 / Accepted: 9 September 2019 / Published: 18 September 2019
(This article belongs to the Special Issue Biosensors Based on Nanostructure Materials)
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Abstract

As a tumor marker, carbohydrate antigen 24-2 (CA242) is a highly accurate and specific diagnostic indicator for monitoring pancreatic and colorectal cancers. The goal of this study was to create a novel label-free electrochemical immunosensor using a nanocomposite glassy carbon electrode for the detection of CA242. Graphene oxide (GO) and polyvinyl pyrrolidone were chosen as the dopants for the preparation of a high-performance reduced-GO-gold-palladium (rGO-Au-Pd) nanocomposite. RGO-Au-Pd was characterized using X-ray diffraction and transmission electron microscopy, revealing that the material exhibited superior electrochemical redox activity and electron transfer ability. The effects of the synthesis method, material concentration, reduction cycle, and pH were investigated to optimize the performance of the immunosensor. As a result of the catalytic activity and biocompatibility of rGO-Au-Pd, the prepared CA242 immunosensor displayed a wide linear range of detection from 0.001 U/mL to 10,000 U/mL with a detection limit of 1.54 × 10−3 U/mL and a sensitivity of 4.24 μA (log10CCA242)−1. More importantly, the immunosensor exhibited satisfactory reproducibility and selectivity when detected CA242 in PBS or human serum. The results of our study provide a platform for the development of novel bioassays for use in early cancer diagnosis and promote the application of biosensing technology in the medical field. View Full-Text
Keywords: CA242; tumor marker; rGO-Au-Pd nanocomposite; electrochemical immunosensor; human serum CA242; tumor marker; rGO-Au-Pd nanocomposite; electrochemical immunosensor; human serum
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MDPI and ACS Style

Du, X.; Zheng, X.; Zhang, Z.; Wu, X.; Sun, L.; Zhou, J.; Liu, M. A Label-Free Electrochemical Immunosensor for Detection of the Tumor Marker CA242 Based on Reduced Graphene Oxide-Gold-Palladium Nanocomposite. Nanomaterials 2019, 9, 1335. https://doi.org/10.3390/nano9091335

AMA Style

Du X, Zheng X, Zhang Z, Wu X, Sun L, Zhou J, Liu M. A Label-Free Electrochemical Immunosensor for Detection of the Tumor Marker CA242 Based on Reduced Graphene Oxide-Gold-Palladium Nanocomposite. Nanomaterials. 2019; 9(9):1335. https://doi.org/10.3390/nano9091335

Chicago/Turabian Style

Du, Xin, Xiaodi Zheng, Zhenhua Zhang, Xiaofan Wu, Lei Sun, Jun Zhou, and Min Liu. 2019. "A Label-Free Electrochemical Immunosensor for Detection of the Tumor Marker CA242 Based on Reduced Graphene Oxide-Gold-Palladium Nanocomposite" Nanomaterials 9, no. 9: 1335. https://doi.org/10.3390/nano9091335

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