Enzymatic Conversion of Oleuropein to Hydroxytyrosol Using Immobilized β-Glucosidase on Porous Carbon Cuboids
AbstractIn the present study, we developed novel β-glucosidase-based nano-biocatalysts for the bioconversion of oleuropein to hydroxytyrosol. Using non-covalent or covalent immobilization approaches, β-glucosidases from almonds and Thermotoga maritima were attached for the first time on oxidized and non-oxidized porous carbon cuboids (PCC). Various methods were used for the characterization of the bio-nanoconjugates, such as Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and fluorescence spectroscopy. The oxidation state of the nanο-support and the immobilization procedure play a key role for the immobilization efficiency or the catalytic activity of the immobilized β-glucosidases. The nano-biocatalysts were successfully used for the hydrolysis of oleuropein, which leads to the formation of its bioactive derivative, hydroxytyrosol (up to 2.4 g L−1), which is a phenolic compound with numerous health benefits. The bio-nanoconjugates exhibited high thermal and operational stability (up to 240 hours of repeated use), which indicated that they are efficient tools for various bio-transformations.
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Chatzikonstantinou, A.V.; Gkantzou, E.; Thomou, E.; Chalmpes, N.; Lyra, K.-M.; Kontogianni, V.G.; Spyrou, K.; Patila, M.; Gournis, D.; Stamatis, H. Enzymatic Conversion of Oleuropein to Hydroxytyrosol Using Immobilized β-Glucosidase on Porous Carbon Cuboids. Nanomaterials 2019, 9, 1166.
Chatzikonstantinou AV, Gkantzou E, Thomou E, Chalmpes N, Lyra K-M, Kontogianni VG, Spyrou K, Patila M, Gournis D, Stamatis H. Enzymatic Conversion of Oleuropein to Hydroxytyrosol Using Immobilized β-Glucosidase on Porous Carbon Cuboids. Nanomaterials. 2019; 9(8):1166.Chicago/Turabian Style
Chatzikonstantinou, Alexandra V.; Gkantzou, Elena; Thomou, Eleni; Chalmpes, Nikolaos; Lyra, Kyriaki-Marina; Kontogianni, Vasiliki G.; Spyrou, Konstantinos; Patila, Michaela; Gournis, Dimitrios; Stamatis, Haralambos. 2019. "Enzymatic Conversion of Oleuropein to Hydroxytyrosol Using Immobilized β-Glucosidase on Porous Carbon Cuboids." Nanomaterials 9, no. 8: 1166.
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