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Nanomaterials 2019, 9(4), 610; https://doi.org/10.3390/nano9040610

Nanoparticles Equipped with α2,8-Linked Sialic Acid Chains Inhibit the Release of Neutrophil Extracellular Traps

1
Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
2
Faculty of Medicine, Institute of Biochemistry, Justus-Liebig-University, Friedrichstrasse 24, 35392 Giessen, Germany
*
Author to whom correspondence should be addressed.
Received: 5 February 2019 / Revised: 18 March 2019 / Accepted: 2 April 2019 / Published: 12 April 2019
(This article belongs to the Special Issue Biomedical Applications of Nanotechnology)
PDF [2267 KB, uploaded 12 April 2019]

Abstract

Neutrophils can combat the invasion of pathogens by the formation of neutrophil extracellular traps (NETs). The NET mechanism is not only an effective tool for combating pathogens, but is also associated with diseases. Therefore, NETs are a potential target for combating pathologies, such as cystic fibrosis and thrombosis. We investigated the potential of nanoparticles, which were modified with α2,8-linked sialic acid chains, to modulate NET release during phorbol myristate acetate stimulation. Interestingly, when these nanoparticles were applied, the formation of reactive oxygen species was partly inhibited and the release of NET was counteracted. However, although the release of NET fibers was prevented, the nuclei still lost their characteristic segmented structure and became swollen, indicating that only the release, and not complete activation was suppressed. Intriguingly, coincubation of α2,8-sialylated particles with free sialic acid chains prevented the outlined inhibitory effects. Thus, the sialic acid chains must be attached to a linker molecule to generate an active bioconjugate that is able to inhibit the release of NET.
Keywords: neutrophil; NETosis; reactive oxygen species (ROS); innate immunity; sialic acids; siglecs; polysaccharide neutrophil; NETosis; reactive oxygen species (ROS); innate immunity; sialic acids; siglecs; polysaccharide
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Bornhöfft, K.F.; Viergutz, T.; Kühnle, A.; Galuska, S.P. Nanoparticles Equipped with α2,8-Linked Sialic Acid Chains Inhibit the Release of Neutrophil Extracellular Traps. Nanomaterials 2019, 9, 610.

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