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Open AccessArticle

A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring

1
Department of Mechanical Engineering, Graduate School, Kyungpook National University, Daegu 41566, Korea
2
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea
3
Department of Mechanical Engineering, Korea Polytechnic University, Siheung 15073, Korea
4
Department of Anatomy, Pusan National University School of Medicine, Yangsan 50612, Korea
5
Department of Pharmacology, Ajou University School of Medicine, Suwon 16499, Korea
6
School of Mechanical Engineering, Kyungpook National University, Daegu 41566, Korea
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(4), 588; https://doi.org/10.3390/nano9040588
Received: 20 February 2019 / Revised: 27 March 2019 / Accepted: 1 April 2019 / Published: 10 April 2019
(This article belongs to the Special Issue Electrospun Nanofibers for Biomedical Applications)
Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days. View Full-Text
Keywords: nanofibers; microfluidic chip; electrospinning; live assay; hepatocellular carcinoma cells nanofibers; microfluidic chip; electrospinning; live assay; hepatocellular carcinoma cells
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Kim, J.H.; Park, J.Y.; Jin, S.; Yoon, S.; Kwak, J.-Y.; Jeong, Y.H. A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring. Nanomaterials 2019, 9, 588.

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