A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring
AbstractRecently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days. View Full-Text
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Kim, J.H.; Park, J.Y.; Jin, S.; Yoon, S.; Kwak, J.-Y.; Jeong, Y.H. A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring. Nanomaterials 2019, 9, 588.
Kim JH, Park JY, Jin S, Yoon S, Kwak J-Y, Jeong YH. A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring. Nanomaterials. 2019; 9(4):588.Chicago/Turabian Style
Kim, Jeong H.; Park, Ju Y.; Jin, Songwan; Yoon, Sik; Kwak, Jong-Young; Jeong, Young H. 2019. "A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring." Nanomaterials 9, no. 4: 588.
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