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Nanomaterials 2019, 9(4), 588;

A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring

Department of Mechanical Engineering, Graduate School, Kyungpook National University, Daegu 41566, Korea
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea
Department of Mechanical Engineering, Korea Polytechnic University, Siheung 15073, Korea
Department of Anatomy, Pusan National University School of Medicine, Yangsan 50612, Korea
Department of Pharmacology, Ajou University School of Medicine, Suwon 16499, Korea
School of Mechanical Engineering, Kyungpook National University, Daegu 41566, Korea
Author to whom correspondence should be addressed.
Received: 20 February 2019 / Revised: 27 March 2019 / Accepted: 1 April 2019 / Published: 10 April 2019
(This article belongs to the Special Issue Electrospun Nanofibers for Biomedical Applications)
PDF [3901 KB, uploaded 10 April 2019]


Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days. View Full-Text
Keywords: nanofibers; microfluidic chip; electrospinning; live assay; hepatocellular carcinoma cells nanofibers; microfluidic chip; electrospinning; live assay; hepatocellular carcinoma cells

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Kim, J.H.; Park, J.Y.; Jin, S.; Yoon, S.; Kwak, J.-Y.; Jeong, Y.H. A Microfluidic Chip Embracing a Nanofiber Scaffold for 3D Cell Culture and Real-Time Monitoring. Nanomaterials 2019, 9, 588.

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