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Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor

Department of Bioengineering, Drug Design, Development and Delivery (4D) Laboratory, Clemson University, Clemson, SC 29634-0905, USA
Department of Chemical Engineering, Wonkwang University, 460, Iksandae-ro, Iksan, Jeonbuk 54538, Korea
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(4), 573;
Received: 11 February 2019 / Revised: 30 March 2019 / Accepted: 30 March 2019 / Published: 8 April 2019
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Spinal cord tumors (SCT) are uncommon neoplasms characterized by irregular growth of tissue inside the spinal cord that can result in non-mechanical back pain. Current treatments for SCT include surgery, radiation therapy, and chemotherapy, but these conventional therapies have many limitations. Suicide gene therapy using plasmid encoding herpes simplex virus-thymidine kinase (pHSV-TK) and ganciclovir (GCV) has been an alternative approach to overcome the limitations of current therapies. However, there is a need to develop a carrier that can deliver both pHSV-TK and GCV for improving therapeutic efficacy. Our group developed a cationic, amphiphilic copolymer, poly (lactide-co-glycolide) -graft-polyethylenimine (PgP), and demonstrated its efficacy as a drug and gene carrier in both cell culture studies and animal models. In this study, we evaluated PgP as a gene carrier and demonstrate that PgP can efficiently deliver reporter genes, pGFP in rat glioma (C6) cells in vitro, and pβ-gal in a rat T5 SCT model in vivo. We also show that PgP/pHSV-TK with GCV treatment showed significantly higher anticancer activity in C6 cells compared to PgP/pHSV-TK without GCV treatment. Finally, we demonstrate that PgP/pHSV-TK with GCV treatment increases the suicide effect and apoptosis of tumor cells and reduces tumor size in a rat T5 SCT model. View Full-Text
Keywords: suicide gene therapy; non-viral gene delivery; ganciclovir; spinal cord tumor suicide gene therapy; non-viral gene delivery; ganciclovir; spinal cord tumor

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Gwak, S.-J.; Lee, J.S. Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor. Nanomaterials 2019, 9, 573.

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