Next Article in Journal
Polyvinylpyrrolidone Nanofibers Encapsulating an Anhydrous Preparation of Fluorescent SiO2–Tb3+ Nanoparticles
Next Article in Special Issue
Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor
Previous Article in Journal
Preparation, Characterization and Properties of Porous PLA/PEG/Curcumin Composite Nanofibers for Antibacterial Application
Previous Article in Special Issue
Structural and Functional Stability of DNA Nanopores in Biological Media

Out-of-Plane Aptamer Functionalization of RNA Three-Helix Tiles

Physik-Department E14, Technische Universität München, 85748 Garching, Germany
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C, Denmark
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(4), 507;
Received: 16 February 2019 / Revised: 23 March 2019 / Accepted: 27 March 2019 / Published: 2 April 2019
Co-transcriptionally folding RNA nanostructures have great potential as biomolecular scaffolds, which can be used to organize small molecules or proteins into spatially ordered assemblies. Here, we develop an RNA tile composed of three parallel RNA double helices, which can associate into small hexagonal assemblies via kissing loop interactions between its two outer helices. The inner RNA helix is modified with an RNA motif found in the internal ribosome entry site (IRES) of the hepatitis C virus (HCV), which provides a 90° bend. This modification is used to functionalize the RNA structures with aptamers pointing perpendicularly away from the tile plane. We demonstrate modifications with the fluorogenic malachite green and Spinach aptamers as well with the protein-binding PP7 and streptavidin aptamers. The modified structures retain the ability to associate into larger assemblies, representing a step towards RNA hybrid nanostructures extending in three dimensions. View Full-Text
Keywords: RNA nanotechnology; aptamers; cotranscriptional folding RNA nanotechnology; aptamers; cotranscriptional folding
Show Figures

Figure 1

MDPI and ACS Style

Chopra, A.; Sagredo, S.; Grossi, G.; Andersen, E.S.; Simmel, F.C. Out-of-Plane Aptamer Functionalization of RNA Three-Helix Tiles. Nanomaterials 2019, 9, 507.

AMA Style

Chopra A, Sagredo S, Grossi G, Andersen ES, Simmel FC. Out-of-Plane Aptamer Functionalization of RNA Three-Helix Tiles. Nanomaterials. 2019; 9(4):507.

Chicago/Turabian Style

Chopra, Aradhana, Sandra Sagredo, Guido Grossi, Ebbe S. Andersen, and Friedrich C. Simmel. 2019. "Out-of-Plane Aptamer Functionalization of RNA Three-Helix Tiles" Nanomaterials 9, no. 4: 507.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop