Next Article in Journal
Effects of Interface Induced Natural Strains on Magnetic Properties of FeRh
Next Article in Special Issue
Smart-Responsive Nucleic Acid Nanoparticles (NANPs) with the Potential to Modulate Immune Behavior
Previous Article in Journal
Effects of Laser Plasma Formation on Quasi-Phase Matching of High-Order Harmonics from Nanoparticles and Atoms
Previous Article in Special Issue
Out-of-Plane Aptamer Functionalization of RNA Three-Helix Tiles
Article Menu

Export Article

Open AccessArticle
Nanomaterials 2019, 9(4), 573; https://doi.org/10.3390/nano9040573

Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor

1
Department of Bioengineering, Drug Design, Development and Delivery (4D) Laboratory, Clemson University, Clemson, SC 29634-0905, USA
2
Department of Chemical Engineering, Wonkwang University, 460, Iksandae-ro, Iksan, Jeonbuk 54538, Korea
*
Author to whom correspondence should be addressed.
Received: 11 February 2019 / Revised: 30 March 2019 / Accepted: 30 March 2019 / Published: 8 April 2019
  |  
PDF [4555 KB, uploaded 19 April 2019]
  |  

Abstract

Spinal cord tumors (SCT) are uncommon neoplasms characterized by irregular growth of tissue inside the spinal cord that can result in non-mechanical back pain. Current treatments for SCT include surgery, radiation therapy, and chemotherapy, but these conventional therapies have many limitations. Suicide gene therapy using plasmid encoding herpes simplex virus-thymidine kinase (pHSV-TK) and ganciclovir (GCV) has been an alternative approach to overcome the limitations of current therapies. However, there is a need to develop a carrier that can deliver both pHSV-TK and GCV for improving therapeutic efficacy. Our group developed a cationic, amphiphilic copolymer, poly (lactide-co-glycolide) -graft-polyethylenimine (PgP), and demonstrated its efficacy as a drug and gene carrier in both cell culture studies and animal models. In this study, we evaluated PgP as a gene carrier and demonstrate that PgP can efficiently deliver reporter genes, pGFP in rat glioma (C6) cells in vitro, and pβ-gal in a rat T5 SCT model in vivo. We also show that PgP/pHSV-TK with GCV treatment showed significantly higher anticancer activity in C6 cells compared to PgP/pHSV-TK without GCV treatment. Finally, we demonstrate that PgP/pHSV-TK with GCV treatment increases the suicide effect and apoptosis of tumor cells and reduces tumor size in a rat T5 SCT model. View Full-Text
Keywords: suicide gene therapy; non-viral gene delivery; ganciclovir; spinal cord tumor suicide gene therapy; non-viral gene delivery; ganciclovir; spinal cord tumor
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Gwak, S.-J.; Lee, J.S. Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor. Nanomaterials 2019, 9, 573.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top