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Open AccessArticle

Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor

1
Department of Bioengineering, Drug Design, Development and Delivery (4D) Laboratory, Clemson University, Clemson, SC 29634-0905, USA
2
Department of Chemical Engineering, Wonkwang University, 460, Iksandae-ro, Iksan, Jeonbuk 54538, Korea
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(4), 573; https://doi.org/10.3390/nano9040573
Received: 11 February 2019 / Revised: 30 March 2019 / Accepted: 30 March 2019 / Published: 8 April 2019
Spinal cord tumors (SCT) are uncommon neoplasms characterized by irregular growth of tissue inside the spinal cord that can result in non-mechanical back pain. Current treatments for SCT include surgery, radiation therapy, and chemotherapy, but these conventional therapies have many limitations. Suicide gene therapy using plasmid encoding herpes simplex virus-thymidine kinase (pHSV-TK) and ganciclovir (GCV) has been an alternative approach to overcome the limitations of current therapies. However, there is a need to develop a carrier that can deliver both pHSV-TK and GCV for improving therapeutic efficacy. Our group developed a cationic, amphiphilic copolymer, poly (lactide-co-glycolide) -graft-polyethylenimine (PgP), and demonstrated its efficacy as a drug and gene carrier in both cell culture studies and animal models. In this study, we evaluated PgP as a gene carrier and demonstrate that PgP can efficiently deliver reporter genes, pGFP in rat glioma (C6) cells in vitro, and pβ-gal in a rat T5 SCT model in vivo. We also show that PgP/pHSV-TK with GCV treatment showed significantly higher anticancer activity in C6 cells compared to PgP/pHSV-TK without GCV treatment. Finally, we demonstrate that PgP/pHSV-TK with GCV treatment increases the suicide effect and apoptosis of tumor cells and reduces tumor size in a rat T5 SCT model. View Full-Text
Keywords: suicide gene therapy; non-viral gene delivery; ganciclovir; spinal cord tumor suicide gene therapy; non-viral gene delivery; ganciclovir; spinal cord tumor
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MDPI and ACS Style

Gwak, S.-J.; Lee, J.S. Suicide Gene Therapy By Amphiphilic Copolymer Nanocarrier for Spinal Cord Tumor. Nanomaterials 2019, 9, 573.

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