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Open AccessCommunication

Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model

1
School of Materials, Faculty of Science and Engineering, University of Manchester, Manchester M13 9PL, UK
2
Department of Physiology, Anatomy and Genetics, South Parks Road, University of Oxford, Oxford OX1 3QX, UK
3
Division of Cell Matrix Biology and Regenerative Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PL, UK
4
Department of Eye and Vision Science, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool L7 8TX, UK
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(4), 522; https://doi.org/10.3390/nano9040522
Received: 26 February 2019 / Revised: 15 March 2019 / Accepted: 21 March 2019 / Published: 3 April 2019
(This article belongs to the Special Issue Stem Cells and Nanotechnology)
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Abstract

This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected by both stimuli over a seven-day period. Cells were orientated parallel to the underlying fibre direction irrespective of environment (electrospun 2D fibre sheet or laminate 2D sheet with collagen gel layer). Application of cyclical tensile force (5% strain, 1 Hz, 1 h per day) encouraged hMSCs to remain at the fibre/gel interface, whereas cells cultured in static conditions migrated from the interface to the upper hydrogel layer. Depending on the stimulus applied, hMSCs presented an up-regulation in gene expression, indicative of several cell lineages, with those cultured at the interface and physically stimulated expressing markers indicative of angiogenesis, osteogenesis, and tenogenesis. This study highlights the importance of developing biomaterial scaffolds with environmental cues to specifically drive cells towards the tissue intended for bioengineering. View Full-Text
Keywords: electrospinning; hydrogels; composites; human mesenchymal stem cells; extracellular matrix; mechanical stimulation electrospinning; hydrogels; composites; human mesenchymal stem cells; extracellular matrix; mechanical stimulation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Kumar, D.; Cain, S.A.; Bosworth, L.A. Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model. Nanomaterials 2019, 9, 522.

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