Next Article in Journal
Tailoring Upconversion and Morphology of Yb/Eu Doped Y2O3 Nanostructures by Acid Composition Mediation
Next Article in Special Issue
Optimization of the Split-Spinach Aptamer for Monitoring Nanoparticle Assembly Involving Multiple Contiguous RNAs
Previous Article in Journal
The Contribution of Magnetic Nanoparticles to Ferrogel Biophysical Properties
Previous Article in Special Issue
Vectors for Glioblastoma Gene Therapy: Viral & Non-Viral Delivery Strategies
Open AccessReview

Intracellular Imaging with Genetically Encoded RNA-Based Molecular Sensors

Department of Chemistry, University of Massachusetts Amherst, Amherst, MA 01003, USA
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(2), 233;
Received: 22 January 2019 / Revised: 5 February 2019 / Accepted: 5 February 2019 / Published: 8 February 2019
Genetically encodable sensors have been widely used in the detection of intracellular molecules ranging from metal ions and metabolites to nucleic acids and proteins. These biosensors are capable of monitoring in real-time the cellular levels, locations, and cell-to-cell variations of the target compounds in living systems. Traditionally, the majority of these sensors have been developed based on fluorescent proteins. As an exciting alternative, genetically encoded RNA-based molecular sensors (GERMS) have emerged over the past few years for the intracellular imaging and detection of various biological targets. In view of their ability for the general detection of a wide range of target analytes, and the modular and simple design principle, GERMS are becoming a popular choice for intracellular analysis. In this review, we summarize different design principles of GERMS based on various RNA recognition modules, transducer modules, and reporting systems. Some recent advances in the application of GERMS for intracellular imaging are also discussed. With further improvement in biostability, sensitivity, and robustness, GERMS can potentially be widely used in cell biology and biotechnology. View Full-Text
Keywords: RNA aptamers; biosensors; live-cell imaging; fluorogenic RNA; riboswitch; ribozyme RNA aptamers; biosensors; live-cell imaging; fluorogenic RNA; riboswitch; ribozyme
Show Figures

Figure 1

MDPI and ACS Style

Sun, Z.; Nguyen, T.; McAuliffe, K.; You, M. Intracellular Imaging with Genetically Encoded RNA-Based Molecular Sensors. Nanomaterials 2019, 9, 233.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop