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Nanomaterials 2019, 9(2), 223; https://doi.org/10.3390/nano9020223

Using N-doped Carbon Dots Prepared Rapidly by Microwave Digestion as Nanoprobes and Nanocatalysts for Fluorescence Determination of Ultratrace Isocarbophos with Label-Free Aptamers

1,2
,
1,2
,
1,2,*
,
1,2
and
1,2,*
1
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Guilin 541004, China
2
Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China
*
Authors to whom correspondence should be addressed.
Received: 5 January 2019 / Revised: 30 January 2019 / Accepted: 5 February 2019 / Published: 7 February 2019
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Abstract

The strongly fluorescent and highly catalytic N-doped carbon dots (CDN) were rapidly prepared by a microwave irradiation procedure and were characterized by electron microscopy (EM), laser scattering, infrared spectroscopy (IR), and by their fluorescence spectrum. It was found that the CDN had a strong catalytic effect on the fluorescence reaction of 3,3′,5,5′-tetramethylbenzidine hydroxide ((TMB)–H2O2) which produced the oxidation product of TMB (TMBOX) with strong fluorescence at 406 nm. The aptamer (Apt) was adsorbed on the CDN surfaces which weakened the fluorescence intensity due to the inhibition of catalytic activity. When the target molecule isocarbophos (IPS) was added, it reacted with the Apt to form a stable conjugate and free CDN which restored the catalytic activity to enhance the fluorescence. Using TMBOX as a fluorescent probe, a highly sensitive nanocatalytic method for determination of 0.025–1.5 μg/L IPS was established with a detection limit of 0.015 μg/L. Coupling the CDN fluorescent probe with the Apt–IPS reaction, a new CD fluorescence method was established for the simple and rapid determination of 0.25–1.5 μg/L IPS with a detection limit of 0.11 μg/L. View Full-Text
Keywords: carbon dot catalysis; TMB; fluorescence; aptamer; isocarbophos carbon dot catalysis; TMB; fluorescence; aptamer; isocarbophos
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Li, X.; Jiang, X.; Liu, Q.; Liang, A.; Jiang, Z. Using N-doped Carbon Dots Prepared Rapidly by Microwave Digestion as Nanoprobes and Nanocatalysts for Fluorescence Determination of Ultratrace Isocarbophos with Label-Free Aptamers. Nanomaterials 2019, 9, 223.

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