Next Article in Journal
Soy Protein Isolate-Phosphatidylcholine Nanoemulsions Prepared Using High-Pressure Homogenization
Next Article in Special Issue
Iron Oxide Colloidal Nanoclusters as Theranostic Vehicles and Their Interactions at the Cellular Level
Previous Article in Journal
Mechanical and Physicochemical Properties of Newly Formed ZnO-PMMA Nanocomposites for Denture Bases
Previous Article in Special Issue
Bare Iron Oxide Nanoparticles for Magnetic Harvesting of Microalgae: From Interaction Behavior to Process Realization
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessReview

Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy

Department of Radiology, Sismanoglio General Hospital of Attica, Sismanogliou 1, 15126 Marousi, Athens, Greece
LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, Portugal
Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, Aghia Paraskevi, 15310 Athens, Greece
“Vinˇca” Institute of Nuclear Sciences, University of Belgrade, 11351 Belgrade, Serbia
Inorganic Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis, 15784 Zografou, Greece
Institute of Nanoscience and Nanotechnology, NCSR Demokritos, Agia Paraskevi Attikis, 15310 Athens, Greece
Department of Biomedical and Clinical Science “Mario Serio”, University of Florence, 50134 Firenze, Italy
Department of Surgery, Radiology & Ph.M. University of the Basque Country, Bilbao E48940, Spain
NanoTherics Ltd., Studio 3, Unit 3, Silverdale Enterprise Centre Kents Lane, Newcastle under Lyme ST5 6SR, UK
School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, Trinity College Dublin, D02PN40 Dublin, Ireland
Authors to whom correspondence should be addressed.
The authors dedicate this review article to the memory of our dearest friend and colleague Maria De Deus Carvalho, from the University of Lisbon, Portugal.
Nanomaterials 2018, 8(5), 306;
Received: 19 March 2018 / Revised: 22 April 2018 / Accepted: 29 April 2018 / Published: 6 May 2018
(This article belongs to the Special Issue Magnetic Nanoparticles in Biological Applications)
PDF [1752 KB, uploaded 29 May 2018]


Magnetic nanoparticle (MNP)-mediated hyperthermia (MH) coupled with radiation therapy (RT) is a novel approach that has the potential to overcome various practical difficulties encountered in cancer treatment. In this work, we present recommendations for the in vitro and in vivo testing and application of the two treatment techniques. These recommendations were developed by the members of Working Group 3 of COST Action TD 1402: Multifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy (“Radiomag”). The purpose of the recommendations is not to provide definitive answers and directions but, rather, to outline those tests and considerations that a researcher must address in order to perform in vitro and in vivo studies. The recommendations are divided into 5 parts: (a) in vitro evaluation of MNPs; (b) in vitro evaluation of MNP-cell interactions; (c) in vivo evaluation of the MNPs; (d) MH combined with RT; and (e) pharmacokinetic studies of MNPs. Synthesis and characterization of the MNPs, as well as RT protocols, are beyond the scope of this work. View Full-Text
Keywords: animal models; biodistribution; in vitro assays; in vivo evaluation; magnetic nanoparticles; pharmacokinetics animal models; biodistribution; in vitro assays; in vivo evaluation; magnetic nanoparticles; pharmacokinetics

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Spirou, S.V.; Costa Lima, S.A.; Bouziotis, P.; Vranješ-Djurić, S.; Efthimiadou, E.Κ.; Laurenzana, A.; Barbosa, A.I.; Garcia-Alonso, I.; Jones, C.; Jankovic, D.; Gobbo, O.L. Recommendations for In Vitro and In Vivo Testing of Magnetic Nanoparticle Hyperthermia Combined with Radiation Therapy. Nanomaterials 2018, 8, 306.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top