The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Raw Materials
2.2. Fabrication of Porous Scaffolds
2.3. Cytotoxicity Assay
2.4. In Vitro Degradation
2.5. Characterization
2.5.1. Water Absorption and Weight Loss
2.5.2. SEM Analysis
2.5.3. DSC Analysis
3. Results and Discussion
3.1. Cytotoxicity
3.2. In Vitro Degradation
3.2.1. Mass Loss and Molecular Weight
3.2.2. Water Uptake
3.2.3. pH
3.2.4. Thermal Analysis (DSC)
3.2.5. FTIR
3.2.6. SEM
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Degradation Time (Weeks) | PLGA 53/47 | |
---|---|---|
0% nHA | 30% nHA | |
Weight-Average Molecular Weight (Mw) | ||
0 | 94,800 | 94,800 |
1 | 29,916 | 58,670 |
2 | - | 35,425 |
3 | 890.7 | 23,106 |
4 | - | 23,811 |
6 | - | 22,141 |
8 | - | 22,936 |
Number-Average Molecular Weight (Mn) | ||
0 | 65,600 | 65,600 |
1 | 16,688 | 33,744 |
2 | - | 10,708 |
3 | 430 | 10,831 |
4 | - | 11,690 |
6 | - | 15,644 |
8 | - | 10,923 |
Polydispersity (I) | ||
0 | 1.445 | 1.445 |
2 | 1.792 | 1.738 |
3 | - | 3.308 |
4 | 2.071 | 2.133 |
6 | - | 1.415 |
8 | - | 2.099 |
Degradation Time (Weeks) | PLGA 75/25 | |||
---|---|---|---|---|
0% nHA | 10% nHA | 30% nHA | 50% nHA | |
Weight-Average Molecular Weight (Mw) | ||||
0 | 86,985 | 86,985 | 86,985 | 86,985 |
2 | 73,311 | 79,604 | 78,758 | 79,455 |
3 | 72,334 | 75,392 | 73,433 | 78,577 |
4 | 66,296 | 75,368 | 69,166 | 72,973 |
6 | 56,212 | 68,749 | 57,371 | 66,178 |
8 | 39,979 | 56,101 | 49,760 | 58,429 |
Number-Average Molecular Weight (Mn) | ||||
0 | 53,533 | 53,533 | 53,533 | 53,533 |
2 | 46,131 | 48,388 | 48,979 | 43,404 |
3 | 42,983 | 43,884 | 44,238 | 45,835 |
4 | 39,782 | 45,490 | 41,345 | 41,808 |
6 | 33,430 | 40,419 | 32,253 | 38,109 |
8 | 19,524 | 31,412 | 29,689 | 33,255 |
Polydispersity (I) | ||||
0 | 1.650 | 1.625 | 1.625 | 1.625 |
2 | 1.589 | 1.645 | 1.608 | 1.830 |
3 | 1.682 | 1.718 | 1.659 | 1.714 |
4 | 1.666 | 1.656 | 1.672 | 1.745 |
6 | 1.681 | 1.701 | 1.778 | 1.736 |
8 | 2.047 | 1.786 | 1.676 | 1.757 |
Degradation Time (Weeks) | Tg (°C) | |||
---|---|---|---|---|
nHA 0% | nHA 10% | nHA 30% | nHA 50% | |
0 | 18.2 | 30.2 | 32.8 | 28.9 |
1 | 44.2 | 33.7 | 34.4 | 30.7 |
2 | N/A | 35.9 | 36.3 | 33.5 |
3 | N/A | 38.4 | 39.4 | 38.8 |
4 | N/A | 35.9 | 43.7 | 43.5 |
6 | N/A | N/A | 40.2 | 40.6 |
8 | N/A | N/A | 40.1 | 40.9 |
Degradation Time (Week) | Tg (°C) | |||
---|---|---|---|---|
nHA 0% | nHA 10% | nHA 30% | nHA 50% | |
0 | 51.5 | 50.4 | 52.9 | 51.0 |
1 | 52.2 | 52.8 | 51.9 | 51.8 |
2 | 51.8 | 52.1 | 51.5 | 53.1 |
3 | 52.6 | 52.2 | 51.9 | 52.5 |
4 | 52.1 | 52.4 | 51.8 | 52.8 |
6 | 52.1 | 52.1 | 51.9 | 52.9 |
8 | 51.0 | 51.9 | 52.1 | 52.5 |
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Díaz, E.; Puerto, I.; Ribeiro, S.; Lanceros-Mendez, S.; Barandiarán, J.M. The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation. Nanomaterials 2017, 7, 173. https://doi.org/10.3390/nano7070173
Díaz E, Puerto I, Ribeiro S, Lanceros-Mendez S, Barandiarán JM. The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation. Nanomaterials. 2017; 7(7):173. https://doi.org/10.3390/nano7070173
Chicago/Turabian StyleDíaz, Esperanza, Igor Puerto, Silvie Ribeiro, Senentxu Lanceros-Mendez, and José Manuel Barandiarán. 2017. "The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation" Nanomaterials 7, no. 7: 173. https://doi.org/10.3390/nano7070173