Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies
Abstract
:1. Introduction
2. Results
2.1. Magnetostatic Calculation
2.2. Transfection of Undifferentiated SH-SY5Y Cells
2.3. Gene Delivery and Prolonged Expression in Primary Hippocampal Neurons on Different Days In Vitro
2.4. Gene Delivery by Oscillating Nanomagnetic Gene Transfection in Primary Cortical Neurons
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. DNA Constructs
4.4. Transfection of Neurons (CN)
4.4.1. Nanomagnetic Gene Transfection
4.4.2. Lipid-Based Transfection
4.5. Flow Cytometry
4.6. Fluorescent Microscopy
4.7. Cell Viability Assay
4.8. Statistics
4.9. Numerical Model and Magnetic Field Calculation
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Subramanian, M.; Tyler, A.-J.; Luther, E.M.; Daniel, E.D.; Lim, J.; Dobson, J. Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies. Nanomaterials 2017, 7, 28. https://doi.org/10.3390/nano7020028
Subramanian M, Tyler A-J, Luther EM, Daniel ED, Lim J, Dobson J. Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies. Nanomaterials. 2017; 7(2):28. https://doi.org/10.3390/nano7020028
Chicago/Turabian StyleSubramanian, Mahendran, Aimee-Jayne Tyler, Eva Maria Luther, Elena Di Daniel, Jenson Lim, and Jon Dobson. 2017. "Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies" Nanomaterials 7, no. 2: 28. https://doi.org/10.3390/nano7020028