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Open AccessArticle

Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology

Section of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
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Author to whom correspondence should be addressed.
Nanomaterials 2017, 7(10), 322; https://doi.org/10.3390/nano7100322
Received: 14 September 2017 / Revised: 7 October 2017 / Accepted: 9 October 2017 / Published: 13 October 2017
(This article belongs to the Special Issue Tissue Engineering and Regenerative Nanomedicine)
Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial–mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel. View Full-Text
Keywords: magnetic nanoparticles; nanotechnology; cell sheet; odontogenic cells; epithelial-mesenchymal interactions; dental enamel regeneration magnetic nanoparticles; nanotechnology; cell sheet; odontogenic cells; epithelial-mesenchymal interactions; dental enamel regeneration
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MDPI and ACS Style

Koto, W.; Shinohara, Y.; Kitamura, K.; Wachi, T.; Makihira, S.; Koyano, K. Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology. Nanomaterials 2017, 7, 322.

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