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Article

Gold Nanobeacons for Tracking Gene Silencing in Zebrafish

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UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
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LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
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Instituto de Medicina Molecular e Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Nanomaterials 2017, 7(1), 10; https://doi.org/10.3390/nano7010010
Received: 20 November 2016 / Revised: 31 December 2016 / Accepted: 5 January 2017 / Published: 11 January 2017
The use of gold nanoparticles for effective gene silencing has demonstrated its potential as a tool for gene expression experiments and for the treatment of several diseases. Here, we used a gold nanobeacon designed to specifically silence the enhanced green fluorescence protein (EGFP) mRNA in embryos of a fli-EGFP transgenic zebrafish line, while simultaneously allowing the tracking and localization of the silencing events via the beacon’s emission. Fluorescence imaging measurements demonstrated a decrease of the EGFP emission with a concomitant increase in the fluorescence of the Au-nanobeacon. Furthermore, microinjection of the Au-nanobeacon led to a negligible difference in mortality and malformations in comparison to the free oligonucleotide, indicating that this system is a biocompatible platform for the administration of gene silencing moieties. Together, these data illustrate the potential of Au-nanobeacons as tools for in vivo zebrafish gene modulation with low toxicity which may be used towards any gene of interest. View Full-Text
Keywords: gold nanobeacons; gold nanoparticles; gene silencing; zebrafish; fli-EGFP gold nanobeacons; gold nanoparticles; gene silencing; zebrafish; fli-EGFP
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MDPI and ACS Style

Cordeiro, M.; Carvalho, L.; Silva, J.; Saúde, L.; Fernandes, A.R.; Baptista, P.V. Gold Nanobeacons for Tracking Gene Silencing in Zebrafish. Nanomaterials 2017, 7, 10. https://doi.org/10.3390/nano7010010

AMA Style

Cordeiro M, Carvalho L, Silva J, Saúde L, Fernandes AR, Baptista PV. Gold Nanobeacons for Tracking Gene Silencing in Zebrafish. Nanomaterials. 2017; 7(1):10. https://doi.org/10.3390/nano7010010

Chicago/Turabian Style

Cordeiro, Milton, Lara Carvalho, Joana Silva, Leonor Saúde, Alexandra R. Fernandes, and Pedro V. Baptista 2017. "Gold Nanobeacons for Tracking Gene Silencing in Zebrafish" Nanomaterials 7, no. 1: 10. https://doi.org/10.3390/nano7010010

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