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Open AccessArticle

A Label-Free Microelectrode Array Based on One-Step Synthesis of Chitosan–Multi-Walled Carbon Nanotube–Thionine for Ultrasensitive Detection of Carcinoembryonic Antigen

by 1,2, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
2
University of Chinese Academy of Science, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Nanomaterials 2016, 6(7), 132; https://doi.org/10.3390/nano6070132
Received: 17 May 2016 / Revised: 1 July 2016 / Accepted: 6 July 2016 / Published: 11 July 2016
Carcinoembryonic antigen (CEA) has been an extensively used tumor marker responsible for clinical early diagnosis of cervical carcinomas, and pancreatic, colorectal, gastric and lung cancer. Combined with micro-electro mechanical system (MEMS) technology, it is important to develop a novel immune microelectrode array (MEA) not only for rapid analysis of serum samples, but also for cell detection in vitro and in vivo. In this work, we depict a simple approach to modify chitosan–multi-walled carbon nanotubes–thionine (CS–MWCNTs–THI) hybrid film through one-step electrochemical deposition and the CS-MWCNTs-THI hybrid films are successfully employed to immobilize anti-CEA for fabricating simple, label-free, and highly sensitive electro-chemical immune MEAs. The detection principle of immune MEA was based on the fact that the increasing formation of the antigen-antibody immunocomplex resulted in the decreased response currents and the relationship between the current reductions with the corresponding CEA concentrations was directly proportional. Experimental results indicated that the label-free MEA had good selectivity and the limit of detection for CEA is 0.5 pg/mL signal to noise ratio (SNR) = 3. A linear calibration plot for the detection of CEA was obtained in a wide concentration range from 1 pg/mL to 100 ng/mL (r = 0.996). This novel MEA has potential applications for detecting CEA for the research on cancer cells and cancer tissue slices as well as for effective early diagnosis. View Full-Text
Keywords: multi-walled carbon nanotube; microelectrode array; carcinoembryonic antigen; one-step synthesis; label-free multi-walled carbon nanotube; microelectrode array; carcinoembryonic antigen; one-step synthesis; label-free
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Xu, H.; Wang, Y.; Wang, L.; Song, Y.; Luo, J.; Cai, X. A Label-Free Microelectrode Array Based on One-Step Synthesis of Chitosan–Multi-Walled Carbon Nanotube–Thionine for Ultrasensitive Detection of Carcinoembryonic Antigen. Nanomaterials 2016, 6, 132.

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