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Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor

1
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
2
School of Electronic and Information Engineering, South China University of Technology, Guangzhou 510640, China
3
The key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
*
Author to whom correspondence should be addressed.
Academic Editor: Ming Su
Nanomaterials 2015, 5(3), 1544-1555; https://doi.org/10.3390/nano5031544
Received: 21 August 2015 / Revised: 10 September 2015 / Accepted: 11 September 2015 / Published: 18 September 2015
(This article belongs to the Special Issue Nanomaterials for Biosensing Applications)
A novel amperometric glucose biosensor was fabricated by layer-by-layer self-assembly of gold nanorods (AuNRs) and glucose oxidase (GOD) onto single-walled carbon nanotubes (SWCNTs)-functionalized three-dimensional sol-gel matrix. A thiolated aqueous silica sol containing SWCNTs was first assembled on the surface of a cleaned Au electrode, and then the alternate self-assembly of AuNRs and GOD were repeated to assemble multilayer films of AuNRs-GOD onto SWCNTs-functionalized silica gel for optimizing the biosensor. Among the resulting glucose biosensors, the four layers of AuNRs-GOD-modified electrode showed the best performance. The sol-SWCNTs-(AuNRs- GOD)4/Au biosensor exhibited a good linear range of 0.01–8 mM glucose, high sensitivity of 1.08 μA/mM, and fast amperometric response within 4 s. The good performance of the proposed glucose biosensor could be mainly attributed to the advantages of the three-dimensional sol-gel matrix and stereo self-assembly films, and the natural features of one-dimensional nanostructure SWCNTs and AuNRs. This study may provide a new facile way to fabricate the enzyme-based biosensor with high performance. View Full-Text
Keywords: biosensor; layer-by-layer; sol-gel; gold nanorods (AuNRs); carbon nanotubes biosensor; layer-by-layer; sol-gel; gold nanorods (AuNRs); carbon nanotubes
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MDPI and ACS Style

Wu, B.; Hou, S.; Miao, Z.; Zhang, C.; Ji, Y. Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor. Nanomaterials 2015, 5, 1544-1555. https://doi.org/10.3390/nano5031544

AMA Style

Wu B, Hou S, Miao Z, Zhang C, Ji Y. Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor. Nanomaterials. 2015; 5(3):1544-1555. https://doi.org/10.3390/nano5031544

Chicago/Turabian Style

Wu, Baoyan, Shihua Hou, Zhiying Miao, Cong Zhang, and Yanhong Ji. 2015. "Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor" Nanomaterials 5, no. 3: 1544-1555. https://doi.org/10.3390/nano5031544

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