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Volume 13
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10.3390/nano13243080
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Article

Ultrasmall ATP-Coated Gold Nanoparticles Specifically Bind to Non-Hybridized Regions in DNA

by
Liat Katrivas
,
Asaf Ben-Menachem
,
Saloni Gupta
and
Alexander B. Kotlyar
*
Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and Nanotechnology Center, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
*
Author to whom correspondence should be addressed.
Nanomaterials 2023, 13(24), 3080; https://doi.org/10.3390/nano13243080
Submission received: 9 November 2023 / Revised: 25 November 2023 / Accepted: 29 November 2023 / Published: 5 December 2023
(This article belongs to the Section Synthesis, Interfaces and Nanostructures)
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Abstract

Here we report the synthesis of ultrasmall (2 nm in diameter) ATP-coated gold nanoparticles, ATP-NPs. ATP-NPs can be enlarged in a predictable manner by the surface-catalyzed reduction of gold ions with ascorbate, yielding uniform gold nanoparticles ranging in size from 2 to 5 nm in diameter. Using atomic force microscopy (AFM), we demonstrate that ATP-NPs can efficiently and selectively bind to a short non-hybridized 5A/5A region (composed of a 5A-nucleotide on each strand of the double helix) inserted into a circular double-stranded plasmid, Puc19. Neither small (1.4 nm in diameter) commercially available nanoparticles nor 5 nm citrate-protected ones are capable of binding to the plasmid. The unique ability to specifically target DNA regions characterized by local structural alterations of the double helix can pave the way for applications of the particles in the detection of genomic DNA regions containing mismatches and mutations that are common for cancer cells.
Keywords: gold nanoparticles; Puc19; DNA–nanoparticle conjugate; AFM; TEM gold nanoparticles; Puc19; DNA–nanoparticle conjugate; AFM; TEM

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MDPI and ACS Style

Katrivas, L.; Ben-Menachem, A.; Gupta, S.; Kotlyar, A.B. Ultrasmall ATP-Coated Gold Nanoparticles Specifically Bind to Non-Hybridized Regions in DNA. Nanomaterials 2023, 13, 3080. https://doi.org/10.3390/nano13243080

AMA Style

Katrivas L, Ben-Menachem A, Gupta S, Kotlyar AB. Ultrasmall ATP-Coated Gold Nanoparticles Specifically Bind to Non-Hybridized Regions in DNA. Nanomaterials. 2023; 13(24):3080. https://doi.org/10.3390/nano13243080

Chicago/Turabian Style

Katrivas, Liat, Asaf Ben-Menachem, Saloni Gupta, and Alexander B. Kotlyar. 2023. "Ultrasmall ATP-Coated Gold Nanoparticles Specifically Bind to Non-Hybridized Regions in DNA" Nanomaterials 13, no. 24: 3080. https://doi.org/10.3390/nano13243080

APA Style

Katrivas, L., Ben-Menachem, A., Gupta, S., & Kotlyar, A. B. (2023). Ultrasmall ATP-Coated Gold Nanoparticles Specifically Bind to Non-Hybridized Regions in DNA. Nanomaterials, 13(24), 3080. https://doi.org/10.3390/nano13243080

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