Gold Nanorods as Radiopharmaceutical Carriers: Preparation and Preliminary Radiobiological In Vitro Tests
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials for AuNR Synthesis and Conjugation
2.2. Characterizations
2.3. AuNR Synthesis
2.4. Preparation of Conjugate Nanorods
2.5. Cell Culture
2.6. Irradiation
2.7. Colony-Forming Assay
3. Results
3.1. AuNR Synthesis and Loading Studies
3.2. Synchrotron Radiation-Induced X-ray Photoelectron Spectral (SR-XPS) Studies
3.3. Biological Studies
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CTAB mL (M) | HAuCl4 mL (M) | AgNO3 μL (M) | AA μL (M) | Seed Solution, μL |
---|---|---|---|---|
10 (0.2) | 10 (0.001) | 400 (0.004) | 70 (0.078) | 24 |
Sample | Dose (Gy) | AuNRs (μg/mL) | Cells Plated |
---|---|---|---|
CN | - | - | 400 |
0.1 μg/mL | - | 0.1 | 400 |
0.5 μg/mL | - | 0.5 | 400 |
1 Gy | 1 | - | 400 |
4 Gy | 4 | - | 1000 |
1a | 1 | 0.1 | 900 |
2a | 1 | 0.5 | 2000 |
3a | 4 | 0.1 | 3000 |
4a | 4 | 0.5 | 4000 |
Sample | SF (see Figure 3b) | Sample | SF (see Figure 4b) | Effect of Auger Electrons |
---|---|---|---|---|
CN | 1 ± 0.14 | 0.1 μg/mL 0.5 μg/mL | 1 ± 0.14 1 ± 0.14 | - - |
1 Gy | 0.81 ± 0.14 | 0.1 μg/mL + 1 Gy 0.5 μg/mL + 1 Gy | 0.71 ± 0.10 0.77 ± 0.11 | 0.11 ± 0.13 0.05 ± 0.14 |
4 Gy | 0.45 ± 0.14 | 0.1 μg/mL + 4 Gy 0.5 μg/mL + 4 Gy | 0.37 ± 0.05 0.39 ± 0.05 | 0.08 ± 0.07 0.06 ± 0.07 |
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Binelli, L.; Dini, V.; Amatori, S.; Scotognella, T.; Giordano, A.; De Berardis, B.; Bertelà, F.; Battocchio, C.; Iucci, G.; Fratoddi, I.; et al. Gold Nanorods as Radiopharmaceutical Carriers: Preparation and Preliminary Radiobiological In Vitro Tests. Nanomaterials 2023, 13, 1898. https://doi.org/10.3390/nano13131898
Binelli L, Dini V, Amatori S, Scotognella T, Giordano A, De Berardis B, Bertelà F, Battocchio C, Iucci G, Fratoddi I, et al. Gold Nanorods as Radiopharmaceutical Carriers: Preparation and Preliminary Radiobiological In Vitro Tests. Nanomaterials. 2023; 13(13):1898. https://doi.org/10.3390/nano13131898
Chicago/Turabian StyleBinelli, Ludovica, Valentina Dini, Simone Amatori, Teresa Scotognella, Alessandro Giordano, Barbara De Berardis, Federica Bertelà, Chiara Battocchio, Giovanna Iucci, Ilaria Fratoddi, and et al. 2023. "Gold Nanorods as Radiopharmaceutical Carriers: Preparation and Preliminary Radiobiological In Vitro Tests" Nanomaterials 13, no. 13: 1898. https://doi.org/10.3390/nano13131898