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Article

Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes

Department of Molecules & Materials, MESA+ Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
*
Author to whom correspondence should be addressed.
Present address: Faculté des Sciences et Technologies—Campus Aiguillettes, Université de Lorraine, CNRS, L2CM, UMR 7053, Bvd des Aiguillettes, F-54506 Vandœuvre-lès-Nancy, France.
Academic Editor: Roberta Pinalli
Nanomaterials 2022, 12(2), 242; https://doi.org/10.3390/nano12020242
Received: 25 November 2021 / Revised: 21 December 2021 / Accepted: 22 December 2021 / Published: 13 January 2022
(This article belongs to the Special Issue Nanoparticles on Supramolecular Sensing)
Affinity sensing of nucleic acids is among the most investigated areas in biosensing due to the growing importance of DNA diagnostics in healthcare research and clinical applications. Here, we report a simple electrochemical DNA detection layer, based on poly-l-lysine (PLL), in combination with gold nanoparticles (AuNPs) as a signal amplifier. The layer shows excellent reduction of non-specific binding and thereby high contrast between amplified and non-amplified signals with functionalized AuNPs; the relative change in current was 10-fold compared to the non-amplified signal. The present work may provide a general method for the detection of tumor markers based on electrochemical DNA sensing. View Full-Text
Keywords: AuNPs; DNA sensing; signal amplification; electrochemical biosensor; poly-l-lysine AuNPs; DNA sensing; signal amplification; electrochemical biosensor; poly-l-lysine
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MDPI and ACS Style

Marti, A.; Huskens, J. Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes. Nanomaterials 2022, 12, 242. https://doi.org/10.3390/nano12020242

AMA Style

Marti A, Huskens J. Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes. Nanomaterials. 2022; 12(2):242. https://doi.org/10.3390/nano12020242

Chicago/Turabian Style

Marti, Almudena, and Jurriaan Huskens. 2022. "Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes" Nanomaterials 12, no. 2: 242. https://doi.org/10.3390/nano12020242

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