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Article

In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies

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Department of Nanobiotechnology, Tarbiat Modares University, Tehran 14115-175, Iran
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Bioengineered Particles Group, CIC biomaGUNE, 20014 San Sebastian, Spain
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Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain
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Instituto Biofisika UPV/EHU, CSIC, 48940 Leioa, Spain
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Departamento de Bioquímica y Biología Molecular, UPV/EHU, 48940 Leioa, Spain
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School of Chemistry, College of Science, University of Tehran, Tehran 14174-66191, Iran
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Fachbereich Physik and CHyN, Universität Hamburg, 22607 Hamburg, Germany
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Institute for Chemical Research (IIQ), CSIC-University of Seville, 41092 Sevilla, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Raphaël Schneider
Nanomaterials 2021, 11(8), 1906; https://doi.org/10.3390/nano11081906
Received: 15 June 2021 / Revised: 19 July 2021 / Accepted: 19 July 2021 / Published: 24 July 2021
(This article belongs to the Special Issue Self-Assembled Nanoparticles: Synthesis and Potential Applications)
Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly). View Full-Text
Keywords: nanoparticles; quantum dots; antibody conjugation; fluorination; self-assembly nanoparticles; quantum dots; antibody conjugation; fluorination; self-assembly
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MDPI and ACS Style

Atabakhshi-Kashi, M.; Carril, M.; Mahdavi, H.; Parak, W.J.; Carrillo-Carrion, C.; Khajeh, K. In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies. Nanomaterials 2021, 11, 1906. https://doi.org/10.3390/nano11081906

AMA Style

Atabakhshi-Kashi M, Carril M, Mahdavi H, Parak WJ, Carrillo-Carrion C, Khajeh K. In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies. Nanomaterials. 2021; 11(8):1906. https://doi.org/10.3390/nano11081906

Chicago/Turabian Style

Atabakhshi-Kashi, Mona, Mónica Carril, Hossein Mahdavi, Wolfgang J. Parak, Carolina Carrillo-Carrion, and Khosro Khajeh. 2021. "In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies" Nanomaterials 11, no. 8: 1906. https://doi.org/10.3390/nano11081906

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