Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Synthesis of Nanostructured Lipid Carriers Loaded with DSG and/or Wild Yam
2.2.2. Particle Size Analysis
2.2.3. Electrokinetic Potential Analysis
2.2.4. Morphological Characterization
2.2.5. Differential Scanning Calorimetry Analysis
2.2.6. Encapsulation Efficiency (EE%) and Drug Loading (DL%)
2.2.7. In Vitro Antioxidant Activity of NLC against Long/Short-Life Free Radicals
Chemiluminescence Assay
ABTS Assay
2.2.8. In Vitro Controlled Release
2.2.9. In Vitro Assessment of Cell Viability
Cytotoxicity by MTS Assay
Cytotoxicity by Real-Time Cell Analysis (RTCA)
2.2.10. The Assessment of the Anti-Inflammatory Activity (ELISA Assay)
2.2.11. Statistical Analysis
3. Results and Discussion
3.1. Size and Stability Features of the NLC Loaded with Hydrophilic and Lipophilic Herbal Bioactive
3.2. The Thermal Behaviour and Entrapment Characteristics of Lipid Nanocarriers Loaded with Herbal Actives
3.3. Assigning In Vitro Cytotoxicity to NLC Systems
3.4. In Vitro Antioxidant Activity through TEAC and Chemiluminescence Method
3.5. In Vitro Controlled Release
3.6. In Vitro Anti-Inflammatory Effect of NLC-DSG-Yam
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Type of NLC * | Lipid Phase (10%), g | Surfactant Mixture (2.5%) g | Herbal Bioactives ***, g | |||||
---|---|---|---|---|---|---|---|---|
GMS | CP | EPO/SOY | Polox 188 | Tw 20 | SC | DSG | Yam | |
NLC-1/2 ** | 3.5 | 3.5 | 3.0 | 0.375 | 0.875 | 1.25 | - | - |
NLC-DSG-1/2 | 0.5 | - | ||||||
NLC-Yam-1/2 | - | 0.5 | ||||||
NLC-DSG-Yam-1/2 | 0.5 | 0.5 |
Sample | Tm (°C) | ΔHm (J/g) | IC | Tc (°C) | ΔHc (J/g) |
---|---|---|---|---|---|
NLC-1 | 46.2 | 6.65 | - | 35.8 | 28.7 |
52.2 | 51.08 | 27.67 | |||
NLC-DSG-Yam-1 | 44.2 | 9.46 | - | 37.4 | 21.58 |
53.1 | 64.17 | 34.76 | 42 | 2.935 | |
Lipids bulk 1 (MSG + PC + EPO) | 37.4 | 7.61 | 100 | 37.9 | 16.26 |
48.6 | 18.46 | 41.5 | 1.98 | ||
55.9 | 1.84 | 47.3 | 8.08 | ||
61.7 | 9.01 | - | - | ||
NLC-2 | 39.9 | 8.59 | - | 38.5 | 25.02 |
50 | 58.66 | 44.81 | 43.2 | 4.736 | |
NLC-DSG-Yam-2 | 38.8 | 7.232 | - | 38 | 15.75 |
48.5 | 51.84 | 39.60 | 42.7 | 3.288 | |
Lipids bulk 2 (MSG + PC + SOY) | 47.1 | 13.09 | 100 | 38.8 | 15.82 |
55.5 | 3.8 | 42.4 | 2.8 | ||
62 | 9.51 | 48.1 | 8.169 |
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Iordache, T.-A.; Badea, N.; Mihaila, M.; Crisan, S.; Pop, A.L.; Lacatusu, I. Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action. Nanomaterials 2021, 11, 3035. https://doi.org/10.3390/nano11113035
Iordache T-A, Badea N, Mihaila M, Crisan S, Pop AL, Lacatusu I. Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action. Nanomaterials. 2021; 11(11):3035. https://doi.org/10.3390/nano11113035
Chicago/Turabian StyleIordache, Teodora-Alexandra, Nicoleta Badea, Mirela Mihaila, Simona Crisan, Anca Lucia Pop, and Ioana Lacatusu. 2021. "Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action" Nanomaterials 11, no. 11: 3035. https://doi.org/10.3390/nano11113035