Designing pH-Dependent Systems Based on Nanoscale Calcium Carbonate for the Delivery of an Antitumor Drug
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of CaNPs by Coprecipitation
2.3. Characterization of CaNPs
2.4. Reversible Binding of CaNPs to DOX
2.5. The Cytotoxicity of CaNPs, CaNP-DOX, and DOX
2.6. Statistical Evaluation of Experimental Error
3. Results and Discussion
3.1. Synthesis and Assessment of CaCO3 Properties
3.2. Interaction of DOX with CaNP7
3.3. Cytotoxicity Assays of CaNP7 and Its Composites with DOX
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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# (Figure 4 Panel) | Sample | Special Conditions | D, nm | PDI |
---|---|---|---|---|
1 (A) | CaNP1 | - | 339 ± 4 | 0.20 ± 0.01 |
2 (B) | CaNP2 | Solvent: isopropanol | 2637 ± 125 | 0.33 ± 0.05 |
3 (C) | CaNP3 | MgCl2, 0.01 M | 278 ± 4 | 0.15 ± 0.01 |
4 (D) | CaNP4 | DMEM, 2 w.% | 333 ± 2 | 0.26 ± 0.01 |
5 (E) | CaNP5 | MgCl2, 0.01 M; DMEM, 2 w.% | 326 ± 6 | 0.26 ± 0.01 |
6 (F) | CaNP6 | MgCl2, 0.05 M; DMEM, 2 w.% | 332 ± 1 | 0.19 ± 0.01 |
7 (G) | CaNP7 | MgCl2, 0.01 M; DMEM, 10 w.% | 249 ± 1 | 0.10 ± 0.01 |
8 (H) | CaNP8 | MgCl2, 0.05 M; DMEM, 10 w.% | 276 ± 4 | 0.10 ± 0.01 |
Sample | IC50, µM |
---|---|
CaNP7–DOX | 0.97 ± 0.04 |
DOX | 2.41 ± 0.02 |
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Popova, V.; Poletaeva, Y.; Pyshnaya, I.; Pyshnyi, D.; Dmitrienko, E. Designing pH-Dependent Systems Based on Nanoscale Calcium Carbonate for the Delivery of an Antitumor Drug. Nanomaterials 2021, 11, 2794. https://doi.org/10.3390/nano11112794
Popova V, Poletaeva Y, Pyshnaya I, Pyshnyi D, Dmitrienko E. Designing pH-Dependent Systems Based on Nanoscale Calcium Carbonate for the Delivery of an Antitumor Drug. Nanomaterials. 2021; 11(11):2794. https://doi.org/10.3390/nano11112794
Chicago/Turabian StylePopova, Victoriya, Yuliya Poletaeva, Inna Pyshnaya, Dmitrii Pyshnyi, and Elena Dmitrienko. 2021. "Designing pH-Dependent Systems Based on Nanoscale Calcium Carbonate for the Delivery of an Antitumor Drug" Nanomaterials 11, no. 11: 2794. https://doi.org/10.3390/nano11112794