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Article

Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen

1
School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece
2
School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(8), 1568; https://doi.org/10.3390/nano10081568
Received: 20 July 2020 / Revised: 6 August 2020 / Accepted: 7 August 2020 / Published: 10 August 2020
(This article belongs to the Special Issue Nanotechnology for Environmental and Biomedical Research)
This study examined the effect of Fe3O4 nanoparticles on boar semen. Beltsville thawing solution without antibiotics was used to extend ejaculates from 5 boars (4 ejaculates/boar). Semen samples of control group (C) and group with Fe3O4 (Fe; 0.192 mg/mL semen) were incubated under routine boar semen storage temperature (17 °C) for 0.5 h and nanoparticles were removed by a magnetic field. Before and after treatment, aliquots of all groups were cultured using standard microbiological methods. The samples after treatment were stored (17 °C) for 48 h and sperm parameters (computer-assisted sperm analyzer (CASA) variables; morphology; viability; hypo-osmotic swelling test (HOST); DNA integrity) were evaluated at storage times 0, 24, 48 h. Semen data were analyzed by a repeated measures mixed model and microbial data with Student’s t-test for paired samples. Regarding CASA parameters, Fe group did not differ from C at any time point. In group C, total motility after 24 h and progressive motility after 48 h of storage decreased significantly compared to 0 h. In group Fe, linearity (LIN) after 48 h and head abnormalities after 24 h of storage increased significantly compared to 0 h. The microbiological results revealed a significant reduction of the bacterial load in group Fe compared to control at both 24 and 48 h. In conclusion, the use of Fe3O4 nanoparticles during semen processing provided a slight anti-microbiological effect with no adverse effects on sperm characteristics. View Full-Text
Keywords: bacterial resistance; boar; microbiological analyses; nanoparticles; semen bacterial resistance; boar; microbiological analyses; nanoparticles; semen
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MDPI and ACS Style

Tsakmakidis, I.A.; Samaras, T.; Anastasiadou, S.; Basioura, A.; Ntemka, A.; Michos, I.; Simeonidis, K.; Karagiannis, I.; Tsousis, G.; Angelakeris, M.; Boscos, C.M. Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen. Nanomaterials 2020, 10, 1568. https://doi.org/10.3390/nano10081568

AMA Style

Tsakmakidis IA, Samaras T, Anastasiadou S, Basioura A, Ntemka A, Michos I, Simeonidis K, Karagiannis I, Tsousis G, Angelakeris M, Boscos CM. Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen. Nanomaterials. 2020; 10(8):1568. https://doi.org/10.3390/nano10081568

Chicago/Turabian Style

Tsakmakidis, Ioannis A., Theodoros Samaras, Sofia Anastasiadou, Athina Basioura, Aikaterini Ntemka, Ilias Michos, Konstantinos Simeonidis, Isidoros Karagiannis, Georgios Tsousis, Mavroeidis Angelakeris, and Constantin M. Boscos 2020. "Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen" Nanomaterials 10, no. 8: 1568. https://doi.org/10.3390/nano10081568

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