Next Article in Journal
One-Step Soft Chemical Synthesis of Magnetite Nanoparticles under Inert Gas Atmosphere. Magnetic Properties and In Vitro Study
Next Article in Special Issue
Assessment of Cytokine-Induced Neutrophil Chemoattractants as Biomarkers for Prediction of Pulmonary Toxicity of Nanomaterials
Previous Article in Journal
Alternating Gyroid Network Structure in an ABC Miktoarm Terpolymer Comprised of Polystyrene and Two Polydienes
Previous Article in Special Issue
Review of Copper and Copper Nanoparticle Toxicity in Fish
 
 
Article

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

1
Moscow Aviation Institute (National Research University), 125993 Moscow, Russia
2
Institute of Physics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia
3
Institute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia
4
Institute of Problems of Chemical Physics RAS, 142432 Chernogolovka, Moscow Region, Russia
5
University of Szeged, H-6720 Szeged, Hungary
6
Siberian Federal University, 660041 Krasnoyarsk, Russia
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(8), 1499; https://doi.org/10.3390/nano10081499
Received: 29 June 2020 / Revised: 25 July 2020 / Accepted: 27 July 2020 / Published: 30 July 2020
(This article belongs to the Special Issue Advances in Nanotoxicology)
Current paper presents biological effects of magnetite nanoparticles (MNPs). Relations of MNP’ characteristics (zeta-potential and hydrodynamic diameters) with effects on bacteria and their enzymatic reactions were the main focus. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc. View Full-Text
Keywords: magnetite nanoparticles; humic acids-coated magnetite nanoparticles; silica-coated magnetite nanoparticles; zeta potential; hydrodynamic diameter; toxicity; bioluminescence; bacterial assay; enzymatic assay; oxidative stress; Photobacterium phosphoreum; NADH:FMN-oxidoreductase; luciferase magnetite nanoparticles; humic acids-coated magnetite nanoparticles; silica-coated magnetite nanoparticles; zeta potential; hydrodynamic diameter; toxicity; bioluminescence; bacterial assay; enzymatic assay; oxidative stress; Photobacterium phosphoreum; NADH:FMN-oxidoreductase; luciferase
Show Figures

Figure 1

MDPI and ACS Style

Bondarenko, L.S.; Kovel, E.S.; Kydralieva, K.A.; Dzhardimalieva, G.I.; Illés, E.; Tombácz, E.; Kicheeva, A.G.; Kudryasheva, N.S. Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions. Nanomaterials 2020, 10, 1499. https://doi.org/10.3390/nano10081499

AMA Style

Bondarenko LS, Kovel ES, Kydralieva KA, Dzhardimalieva GI, Illés E, Tombácz E, Kicheeva AG, Kudryasheva NS. Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions. Nanomaterials. 2020; 10(8):1499. https://doi.org/10.3390/nano10081499

Chicago/Turabian Style

Bondarenko, Lyubov S., Ekaterina S. Kovel, Kamila A. Kydralieva, Gulzhian I. Dzhardimalieva, Erzsébet Illés, Etelka Tombácz, Arina G. Kicheeva, and Nadezhda S. Kudryasheva. 2020. "Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions" Nanomaterials 10, no. 8: 1499. https://doi.org/10.3390/nano10081499

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop