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Article

Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth

by 1,†, 2,3,†, 2,3,4, 5, 2,3,4,6, 1,*, 2,3,4,6,* and 1,2,*
1
Department of Pediatric Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea
2
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea
3
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea
4
UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea
5
Sounth China Center of Craniofacial Stem Cell Research, Sun Yat-sen University, Guangzhou 510055, China
6
Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2020, 10(7), 1373; https://doi.org/10.3390/nano10071373
Received: 9 June 2020 / Revised: 4 July 2020 / Accepted: 9 July 2020 / Published: 14 July 2020
(This article belongs to the Special Issue Nanomaterials and Nanotechnology for Regenerative Medicine)
Nanomaterials can enhance interactions with stem cells for tissue regeneration. This study aimed to investigate the biological effects of tricalcium silicate nanoparticle-containing cement (Biodentine™) during or after setting on stem cells from human exfoliated deciduous teeth (SHED) to mimic clinically relevant situations in which materials are adapted. Specimens were divided into four groups depending on the start of extraction time (during (3, 6 and 12 min) or after setting (24 h)) and extracted in culture medium for 24 h for further physicochemical and biological analysis. After cell viability in serially diluted extracts was evaluated, odontogenic differentiation on SHED was evaluated by ARS staining using nontoxic conditions. A physicochemical analysis of extracts or specimens indicated different Ca ion content, pH, and surface chemistry among groups, supporting the possibility of different biological functionalities depending on the extraction starting conditions. Compared to the ‘after setting’ group, all ‘during setting’ groups showed cytotoxicity on SHED. The during setting groups induced more odontogenic differentiation at the nontoxic concentrations compared to the control. Thus, under clinically simulated extract conditions at nontoxic concentrations, Biodentine™ seemed to be a promising odontoblast differentiating biomaterial that is helpful for dental tissue regeneration. In addition, to simulate clinical situations when nanoparticle-containing cement is adjusted, biological effects during setting need to be considered. View Full-Text
Keywords: calcium silicate-based cement; nanoparticles; stem cells from human exfoliated deciduous teeth; cytotoxicity test; odontogenic differentiation; dental pulp capping; deciduous teeth calcium silicate-based cement; nanoparticles; stem cells from human exfoliated deciduous teeth; cytotoxicity test; odontogenic differentiation; dental pulp capping; deciduous teeth
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MDPI and ACS Style

Jung, Y.; Yoon, J.-Y.; Dev Patel, K.; Ma, L.; Lee, H.-H.; Kim, J.; Lee, J.-H.; Shin, J. Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth. Nanomaterials 2020, 10, 1373. https://doi.org/10.3390/nano10071373

AMA Style

Jung Y, Yoon J-Y, Dev Patel K, Ma L, Lee H-H, Kim J, Lee J-H, Shin J. Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth. Nanomaterials. 2020; 10(7):1373. https://doi.org/10.3390/nano10071373

Chicago/Turabian Style

Jung, Yoonsun, Ji-Young Yoon, Kapil Dev Patel, Lan Ma, Hae-Hyoung Lee, Jongbin Kim, Jung-Hwan Lee, and Jisun Shin. 2020. "Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth" Nanomaterials 10, no. 7: 1373. https://doi.org/10.3390/nano10071373

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