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Article

Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor

1
Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER in Bioengineering, Biomaterials and Nanomedicine and BIST, Campus UAB Bellaterra, 08193 Barcelona, Spain
2
Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, CIBER in Bioengineering, Biomaterials and Nanomedicine c/Jordi Girona 18–26, 08034 Barcelona, Spain
3
Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville and CIBER in Epidemiology and Public Health, 41013 Seville, Spain
4
Institute for Applied Microelectronics (IUMA), University of Las Palmas de Gran Canaria, 35017 Las Palmas, Spain
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Integrated Photonics and Applications Centre, School of Engineering, RMIT University, Melbourne 3001, Australia
6
Department of Medicine, University of Seville, 41013 Seville, Spain
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(6), 1246; https://doi.org/10.3390/nano10061246
Received: 9 June 2020 / Revised: 22 June 2020 / Accepted: 23 June 2020 / Published: 26 June 2020
(This article belongs to the Special Issue The Application of DNA Nanotechnology)
Pneumocystis jirovecii is a fungus responsible for human Pneumocystis pneumonia, one of the most severe infections encountered in immunodepressed individuals. The diagnosis of Pneumocystis pneumonia continues to be challenging due to the absence of specific symptoms in infected patients. Moreover, the standard diagnostic method employed for its diagnosis involves mainly PCR-based techniques, which besides being highly specific and sensitive, require specialized personnel and equipment and are time-consuming. Our aim is to demonstrate an optical biosensor methodology based on surface plasmon resonance to perform such diagnostics in an efficient and decentralized scheme. The biosensor methodology employs poly-purine reverse-Hoogsteen hairpin probes for the detection of the mitochondrial large subunit ribosomal RNA (mtLSU rRNA) gene, related to P. jirovecii detection. The biosensor device performs a real-time and label-free identification of the mtLSU rRNA gene with excellent selectivity and reproducibility, achieving limits of detection of around 2.11 nM. A preliminary evaluation of clinical samples showed rapid, label-free and specific identification of P. jirovecii in human lung fluids such as bronchoalveolar lavages or nasopharyngeal aspirates. These results offer a door for the future deployment of a sensitive diagnostic tool for fast, direct and selective detection of Pneumocystis pneumonia disease. View Full-Text
Keywords: Pneumocystis jirovecii; surface plasmon resonance; optical biosensor; clinical diagnosis; triplex; DNA capture Pneumocystis jirovecii; surface plasmon resonance; optical biosensor; clinical diagnosis; triplex; DNA capture
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MDPI and ACS Style

Calvo-Lozano, O.; Aviñó, A.; Friaza, V.; Medina-Escuela, A.; S. Huertas, C.; Calderón, E.J.; Eritja, R.; Lechuga, L.M. Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor. Nanomaterials 2020, 10, 1246. https://doi.org/10.3390/nano10061246

AMA Style

Calvo-Lozano O, Aviñó A, Friaza V, Medina-Escuela A, S. Huertas C, Calderón EJ, Eritja R, Lechuga LM. Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor. Nanomaterials. 2020; 10(6):1246. https://doi.org/10.3390/nano10061246

Chicago/Turabian Style

Calvo-Lozano, Olalla, Anna Aviñó, Vicente Friaza, Alfonso Medina-Escuela, César S. Huertas, Enrique J. Calderón, Ramón Eritja, and Laura M. Lechuga 2020. "Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor" Nanomaterials 10, no. 6: 1246. https://doi.org/10.3390/nano10061246

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