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DNA-Biofunctionalization of CTAC-Capped Gold Nanocubes

Leibniz-Institute for Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany
Author to whom correspondence should be addressed.
Current address: School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, UK.
Nanomaterials 2020, 10(6), 1119;
Received: 11 May 2020 / Revised: 1 June 2020 / Accepted: 3 June 2020 / Published: 5 June 2020
(This article belongs to the Special Issue Safety and Biocompatibility of Metallic Nanoparticles)
Clinical diagnostics and disease control are fields that strongly depend on technologies for rapid, sensitive, and selective detection of biological or chemical analytes. Nanoparticles have become an integral part in various biomedical detection devices and nanotherapeutics. An increasing focus is laid on gold nanoparticles as they express less cytotoxicity, high stability, and hold unique optical properties with the ability of signal transduction of biological recognition events with enhanced analytical performance. Strong electromagnetic field enhancements can be found in close proximity to the nanoparticle that can be exploited to enhance signals for e.g., metal-enhanced fluorescence or Raman spectroscopy. Even stronger field enhancements can be achieved with sharp-edged nanoparticles, which are synthesized with the help of facet blocking agents, such as cetyltrimethylammonium bromide/chloride (CTAB/CTAC). However, chemical modification of the nanoparticle surface is necessary to reduce the particle’s cytotoxicity, stabilize it against aggregation, and to bioconjugate it with biomolecules to increase its biocompatibility and/or specificity for analytical applications. Here, a reliable two-step protocol following a ligand exchange with bis (p-sulfonatophenyl) phenyl phosphine (BSPP) as the intermediate capping-agent is demonstrated, which results in the reliable biofunctionalization of CTAC-capped gold nanocubes with thiol-modified DNA. The functionalized nanocubes have been characterized regarding their electric potential, plasmonic properties, and stability against high concentrations of NaCl and MgCl2. View Full-Text
Keywords: biofunctionalization; CTAC; shape-anisotropic nanoparticles; gold nanocubes; DNA biofunctionalization; CTAC; shape-anisotropic nanoparticles; gold nanocubes; DNA
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MDPI and ACS Style

Slesiona, N.; Thamm, S.; Stolle, H.L.K.S.; Weißenborn, V.; Müller, P.; Csáki, A.; Fritzsche, W. DNA-Biofunctionalization of CTAC-Capped Gold Nanocubes. Nanomaterials 2020, 10, 1119.

AMA Style

Slesiona N, Thamm S, Stolle HLKS, Weißenborn V, Müller P, Csáki A, Fritzsche W. DNA-Biofunctionalization of CTAC-Capped Gold Nanocubes. Nanomaterials. 2020; 10(6):1119.

Chicago/Turabian Style

Slesiona, Nicole, Sophie Thamm, H. L.K.S. Stolle, Viktor Weißenborn, Philipp Müller, Andrea Csáki, and Wolfgang Fritzsche. 2020. "DNA-Biofunctionalization of CTAC-Capped Gold Nanocubes" Nanomaterials 10, no. 6: 1119.

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