Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us

Krce, Lucija; Šprung, Matilda; Rončević, Tomislav; Maravić, Ana; Čikeš Čulić, Vedrana; Blažeka, Damjan; Krstulović, Nikša; Aviani, Ivica

doi:10.3390/nano10061040

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Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us

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Ivica Aviani

Department of Physics, Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia

Department of Chemistry, Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia

Department of Biology, Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia

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Department of Medical Chemistry and Biochemistry, School of Medicine, University of Split, Šoltanska ulica 2, 21000 Split, Croatia

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Institute of Physics, Bijenička cesta 46, 10000 Zagreb, Croatia

Author to whom correspondence should be addressed.

Nanomaterials 2020, 10(6), 1040; https://doi.org/10.3390/nano10061040

Received: 30 April 2020 / Revised: 26 May 2020 / Accepted: 27 May 2020 / Published: 29 May 2020

(This article belongs to the Special Issue Safety and Biocompatibility of Metallic Nanoparticles)

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Abstract

We aim to elucidate the mode of antibacterial action of the laser-synthesized silver colloid against Escherichia coli. Membrane integrity was studied by flow cytometry, while the strain viability of the treated culture was determined by plating. The spectrofluorometry was used to obtain the time development of the reactive oxygen species (ROS) inside the nanoparticle-treated bacterial cells. An integrated atomic force and bright-field/fluorescence microscopy system enabled the study of the cell morphology, Young modulus, viability, and integrity before and during the treatment. Upon lethal treatment, not all bacterial cells were shown to be permeabilized and have mostly kept their morphology with an indication of cell lysis. Young modulus of untreated cells was shown to be distinctly bimodal, with randomly distributed softer parts, while treated cells exhibited exponential softening of the stiffer parts in time. Silver nanoparticles and bacteria have shown a masking effect on the raw fluorescence signal through absorbance and scattering. The contribution of cellular ROS in the total fluorescence signal was resolved and it was proven that the ROS level inside the lethally treated cells is not significant. It was found that the laser-synthesized silver nanoparticles mode of antibacterial action includes reduction of the cell’s Young modulus in time and subsequently the cell leakage. View Full-Text

Keywords: laser-synthesized nanoparticles; silver nanoparticles; mode of antibacterial action; nano-bio interactions; reactive oxygen species; atomic force microscopy; Young modulus

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MDPI and ACS Style

Krce, L.; Šprung, M.; Rončević, T.; Maravić, A.; Čikeš Čulić, V.; Blažeka, D.; Krstulović, N.; Aviani, I. Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us. Nanomaterials 2020, 10, 1040. https://doi.org/10.3390/nano10061040

AMA Style

Krce L, Šprung M, Rončević T, Maravić A, Čikeš Čulić V, Blažeka D, Krstulović N, Aviani I. Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us. Nanomaterials. 2020; 10(6):1040. https://doi.org/10.3390/nano10061040

Chicago/Turabian Style

Krce, Lucija, Matilda Šprung, Tomislav Rončević, Ana Maravić, Vedrana Čikeš Čulić, Damjan Blažeka, Nikša Krstulović, and Ivica Aviani. 2020. "Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us" Nanomaterials 10, no. 6: 1040. https://doi.org/10.3390/nano10061040

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Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us

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