Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis
by
Tanja Zidarič 1,*
, Marko Milojević 1, Lidija Gradišnik 1, Karin Stana Kleinschek 2,3,4, Uroš Maver 1,5,* and Tina Maver 1,2,*
Tanja Zidarič 1,*, Marko Milojević 1, Lidija Gradišnik 1, Karin Stana Kleinschek 2,3,4, Uroš Maver 1,5,* and Tina Maver 1,2,*
1
Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia
2
Laboratory for Characterization and Processing of Polymers (LCPP), Faculty of Mechanical Engineering, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia
3
Institute of Chemistry and Technology of Biobased Systems, Faculty of Technical Chemistry, Chemical and Process Engineering and Biotechnology, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria
4
Institute of Automation, Faculty of Electrical Engineering and Computer Science, University of Maribor, Koroška cesta 46, 2000 Maribor, Slovenia
5
Department of Pharmacology, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(4), 733; https://doi.org/10.3390/nano10040733
Received: 10 February 2020 / Revised: 1 April 2020 / Accepted: 8 April 2020 / Published: 11 April 2020
(This article belongs to the Special Issue Polysaccharide-Based Nanomaterials and Their Applications)
Limitations in wound management have prompted scientists to introduce bioprinting techniques for creating constructs that can address clinical problems. The bioprinting approach is renowned for its ability to spatially control the three-dimensional (3D) placement of cells, molecules, and biomaterials. These features provide new possibilities to enhance homology to native skin and improve functional outcomes. However, for the clinical value, the development of hydrogel bioink with refined printability and bioactive properties is needed. In this study, we combined the outstanding viscoelastic behavior of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate (ALG), carboxymethyl cellulose (CMC), and encapsulated human-derived skin fibroblasts (hSF) to create a bioink for the 3D bioprinting of a dermis layer. The shear thinning behavior of hSF-laden bioink enables construction of 3D scaffolds with high cell density and homogeneous cell distribution. The obtained results demonstrated that hSF-laden bioink supports cellular activity of hSF (up to 29 days) while offering proper printability in a biologically relevant 3D environment, making it a promising tool for skin tissue engineering and drug testing applications.
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Keywords:
alginate; carboxymethyl cellulose; nanofibrillated cellulose; human-derived skin fibroblasts; 3D cell-laden scaffolds; in vitro skin model
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MDPI and ACS Style
Zidarič, T.; Milojević, M.; Gradišnik, L.; Stana Kleinschek, K.; Maver, U.; Maver, T. Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis. Nanomaterials 2020, 10, 733.
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