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Open AccessArticle

Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence

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Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380494, Chile
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Advanced Center for Chronic Diseases (ACCDiS), Sergio Livingstone 1007, Independencia, Santiago 8380494, Chile
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Laboratorio de Procesos Fotónicos y Electroquímicos, Universidad de Playa Ancha, Playa Ancha 850, Valparaíso, Chile
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Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Beaucheff 851, Santiago 8380494, Chile
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CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine & Department of Organic Chemistry, Marti i Franques 1-11, University of Barcelona (UB), 08028 Barcelona, Spain
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School of Chemistry & Physics, University of KwaZulu-Natal, Durban 4001, South Africa
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Núcleo de Biotecnología Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Valparaíso 2460355, Chile
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Department of Chemistry, Faculty of Sciences, University of Chile, POBox 653, Santiago 8380494, Chile
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Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago 8331010, Chile
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Centro de envejecimiento y regeneración (CARE), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8380494, Chile
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Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(4), 690; https://doi.org/10.3390/nano10040690
Received: 11 March 2020 / Revised: 28 March 2020 / Accepted: 29 March 2020 / Published: 6 April 2020
(This article belongs to the Section Biology and Medicines)
The early detection of the amyloid beta peptide aggregates involved in Alzheimer’s disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods (GNRs) by the surface enhancement fluorescence effect. We synthetized GNRs and modified their surface with HS-PEG-OMe and HS-PEG-COOH and functionalized them with the D1 peptide, which has the capability to selectively bind to amyloid beta peptide. For an in vitro detection of amyloid beta peptide, we co-incubated amyloid beta peptide aggregates with the probe CRANAD-2 and GNR-PEG-D1 observing an increase in the intensity of the fluorescence signal attributed to surface enhancement fluorescence. Furthermore, the surface enhancement fluorescence effect was observed in brain slices of transgenic mice with Alzheimer´s disease co-incubated with CRANAD-2 and GNR-PEG-D1. An increase in the fluorescence signal was observed allowing the detection of aggregates that cannot be detected with the single use of CRANAD-2. Gold nanoparticles allowed an improvement in the detection of the amyloid aggregated by fluorescence in vitro and ex vivo. View Full-Text
Keywords: gold nanorods; CRANAD-2; amyloid beta peptide; SEF; Alzheimer´s disease gold nanorods; CRANAD-2; amyloid beta peptide; SEF; Alzheimer´s disease
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MDPI and ACS Style

Jara-Guajardo, P.; Cabrera, P.; Celis, F.; Soler, M.; Berlanga, I.; Parra-Muñoz, N.; Acosta, G.; Albericio, F.; Guzman, F.; Campos, M.; Alvarez, A.; Morales-Zavala, F.; Kogan, M.J. Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence. Nanomaterials 2020, 10, 690.

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