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Open AccessArticle

Polyethylene Glycol6000/carbon Nanodots as Fluorescent Bioimaging Agents

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Department of Chemical and Materials Engineering, Chang Gung University, Guishan, Taoyuan 33302, Taiwan
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Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan
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Department of Food Science and Biotechnology, Da-Yeh University, Changhua 51591, Taiwan
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Department of Mold and Die Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 80778, Taiwan
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Department of Safety, Health and Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 81164, Taiwan
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Department of SeaFood Science, National Kaohsiung University of Science Kaohsiung 81157, Taiwan
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Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan
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Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996, USA
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Division of Nephrology, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou 33375, Taiwan
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Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(4), 677; https://doi.org/10.3390/nano10040677
Received: 14 March 2020 / Revised: 29 March 2020 / Accepted: 31 March 2020 / Published: 3 April 2020
Photoluminescent nanomaterials have immense potential for use in biological systems due to their excellent fluorescent properties and small size. Traditional semiconductor quantum dots are heavy-metal-based and can be highly toxic to living organisms, besides their poor photostability and low biocompatibility. Nano-sized carbon quantum dots and their surface-modified counterparts have shown improved characteristics for imaging purposes. We used 1,3, 6-trinitropyrene (TNP) and polyethylene glycol6000 (PEG6000) in a hydrothermal method to prepare functional polyethylene glycol6000/carbon nanodots (PEG6000/CDs) and analyzed their potential in fluorescent staining of different types of bacteria. Our results demonstrated that PEG6000/CDs stained the cell pole and septa of gram-positive bacteria B. Subtilis and B. thuringiensis but not those of gram-negative bacteria. The optimal concentration of these composite nanodots was approximately 100 ppm and exposure times varied across different bacteria. The PEG6000/CD composite had better photostability and higher resistance to photobleaching than the commercially available FM4-64. They could emit two wavelengths (red and green) when exposed to two different wavelengths. Therefore, they may be applicable as bioimaging molecules. They can also be used for differentiating different types of bacteria owing to their ability to differentially stain gram-positive and gram-negative bacteria. View Full-Text
Keywords: carbon quantum dots; nanomaterials; bioimaging; photoluminescence; hydrothermal synthesis carbon quantum dots; nanomaterials; bioimaging; photoluminescence; hydrothermal synthesis
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Fu, C.-C.; Wu, C.-Y.; Chien, C.-C.; Hsu, T.-H.; Ou, S.-F.; Chen, S.-T.; Wu, C.-H.; Hsieh, C.-T.; Juang, R.-S.; Hsueh, Y.-H. Polyethylene Glycol6000/carbon Nanodots as Fluorescent Bioimaging Agents. Nanomaterials 2020, 10, 677.

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