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Open AccessArticle

Challenges in Tracking of Fluorochrome-Labelled Nanoparticles in Mice via Whole Body NIRF Imaging

1
Institute for Diagnostic and Interventional Radiology, Jena University Hospital—Friedrich Schiller University Jena, D-07740 Jena, Germany
2
Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany
3
Department of Veterinary Clinical Sciences, Small Animal Clinic, Justus- Liebig-University, D-35390 Gießen, Germany
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(3), 596; https://doi.org/10.3390/nano10030596 (registering DOI)
Received: 28 January 2020 / Revised: 19 March 2020 / Accepted: 20 March 2020 / Published: 24 March 2020
Fluorochrome-labelled iron oxide magnetic nanoparticles (MNP) have been of great help in elucidating biological processes. Here, we used dually-fluorochrome-labelled MNP and studied to what extent fluorescence detection could reflect their fate in living animals. One day after application in mice (200 µmol Fe/kg body weight), the fluorescence of the dye attached to the core (DY-730) was very prominent and in agreement with the increase of iron in the liver and spleen of mice, but inconspicuous at time points thereafter. We attribute this fluorescence behavior to early degradation processes of the MNP´s core in the cellular lysosomal compartment. In contrast, the fluorescence of the dye DY-555 stuck to the PEG coating was not detectable in vivo. In summary, labelling of MNP with dyes at their metallic core could be of help when detecting first incidences of MNP biodegradation in vivo, as opposed to dyes attached to the MNP coating. View Full-Text
Keywords: fluorescence; nanoparticles; iron oxide; nanoparticle tracking; nanoparticle labelling fluorescence; nanoparticles; iron oxide; nanoparticle tracking; nanoparticle labelling
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Gaffron, F.; Tilch, A.; Grüttner, C.; Kowalski, A.; Kramer, M.; Teichgräber, U.; Hilger, I. Challenges in Tracking of Fluorochrome-Labelled Nanoparticles in Mice via Whole Body NIRF Imaging. Nanomaterials 2020, 10, 596.

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