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Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles

1
German Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany
2
Information Technology Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaitherburg, MD 20899-8311, USA
3
Materials Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaitherburg, MD 20899-8311, USA
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(12), 2369; https://doi.org/10.3390/nano10122369
Received: 3 November 2020 / Revised: 26 November 2020 / Accepted: 27 November 2020 / Published: 28 November 2020
(This article belongs to the Special Issue Design, Development, and Production of Nanocarriers and Nanovehicles)
Air–liquid interface (ALI) systems have been widely used in recent years to investigate the inhalation toxicity of many gaseous compounds, chemicals, and nanomaterials and represent an emerging and promising in vitro method to supplement in vivo studies. ALI exposure reflects the physiological conditions of the deep lung more closely to subacute in vivo inhalation scenarios compared to submerged exposure. The comparability of the toxicological results obtained from in vivo and in vitro inhalation data is still challenging. The robustness of ALI exposure scenarios is not yet well understood, but critical for the potential standardization of these methods. We report a cause-and-effect (C&E) analysis of a flow through ALI exposure system. The influence of five different instrumental and physiological parameters affecting cell viability and exposure parameters of a human lung cell line in vitro (exposure duration, relative humidity, temperature, CO2 concentration and flow rate) was investigated. After exposing lung epithelia cells to a CeO2 nanoparticle (NP) aerosol, intracellular CeO2 concentrations reached values similar to those found in a recent subacute rat inhalation study in vivo. This is the first study showing that the NP concentration reached in vitro using a flow through ALI system were the same as those in an in vivo study. View Full-Text
Keywords: air–liquid interface system; inhalation toxicology; nanoparticles; CeO2; standardization; cause-and-effect analysis air–liquid interface system; inhalation toxicology; nanoparticles; CeO2; standardization; cause-and-effect analysis
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MDPI and ACS Style

Leibrock, L.B.; Jungnickel, H.; Tentschert, J.; Katz, A.; Toman, B.; Petersen, E.J.; Bierkandt, F.S.; Singh, A.V.; Laux, P.; Luch, A. Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles. Nanomaterials 2020, 10, 2369. https://doi.org/10.3390/nano10122369

AMA Style

Leibrock LB, Jungnickel H, Tentschert J, Katz A, Toman B, Petersen EJ, Bierkandt FS, Singh AV, Laux P, Luch A. Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles. Nanomaterials. 2020; 10(12):2369. https://doi.org/10.3390/nano10122369

Chicago/Turabian Style

Leibrock, Lars B.; Jungnickel, Harald; Tentschert, Jutta; Katz, Aaron; Toman, Blaza; Petersen, Elijah J.; Bierkandt, Frank S.; Singh, Ajay V.; Laux, Peter; Luch, Andreas. 2020. "Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles" Nanomaterials 10, no. 12: 2369. https://doi.org/10.3390/nano10122369

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