Next Article in Journal
Mechanochemical Synthesis of Nanocrystalline Hydroxyapatite from Ca(H2PO4)2.H2O, CaO, Ca(OH)2, and P2O5 Mixtures
Next Article in Special Issue
Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles
Previous Article in Journal
Femtosecond Double-Pulse Laser Ablation and Deposition of Co-Doped ZnS Thin Films
Previous Article in Special Issue
Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
Article

Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent 19F MRI Tracer

1
Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského náměstí 2, 162 06 Prague 6, Czech Republic
2
Institute of Physics, Faculty of Mathematics and Physics, Charles University in Prague, Ke Karlovu 3, 121 16 Prague 2, Czech Republic
3
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, 128 43 Prague 2, Czech Republic
4
Department of Organic Chemistry, Faculty of Science, Charles University, Hlavova 8, 128 43 Prague 2, Czech Republic
5
Institute of Biophysics and Informatics, Charles University, First Faculty of Medicine, Salmovská 1, 120 00 Prague 2, Czech Republic
6
Department of NMR Spectroscopy, Faculty of Science, Charles University, Hlavova 8, 128 43 Prague 2, Czech Republic
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2020, 10(11), 2231; https://doi.org/10.3390/nano10112231
Received: 25 September 2020 / Revised: 21 October 2020 / Accepted: 5 November 2020 / Published: 10 November 2020
(This article belongs to the Special Issue Frontiers in Nanomaterials for Clinical Imaging and Selective Therapy)
Fluorine-19 MRI is a promising noninvasive diagnostic method. However, the absence of a nontoxic fluorine-19 MRI tracer that does not suffer from poor biodistribution as a result of its strong fluorophilicity is a constant hurdle in the widespread applicability of this otherwise versatile diagnostic technique. The poly[N-(2-hydroxypropyl)methacrylamide]-block-poly[N-(2,2-difluoroethyl)acrylamide] thermoresponsive copolymer was proposed as an alternative fluorine-19 MRI tracer capable of overcoming such shortcomings. In this paper, the internal structure of self-assembled particles of this copolymer was investigated by various methods including 1D and 2D NMR, dynamic light scattering (DLS), small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS). The elucidated structure appears to be that of a nanogel with greatly swollen hydrophilic chains and tightly packed thermoresponsive chains forming a network within the nanogel particles, which become more hydrophobic with increasing temperature. Its capacity to provide a measurable fluorine-19 NMR signal in its aggregated state at human body temperature was also investigated and confirmed. This capacity stems from the different fluorine-19 nuclei relaxation properties compared to those of hydrogen-1 nuclei. View Full-Text
Keywords: fluorine-19; magnetic resonance imaging; self-assembly; diblock copolymer; PHPMA; PDFEA fluorine-19; magnetic resonance imaging; self-assembly; diblock copolymer; PHPMA; PDFEA
Show Figures

Graphical abstract

MDPI and ACS Style

Babuka, D.; Kolouchova, K.; Groborz, O.; Tosner, Z.; Zhigunov, A.; Stepanek, P.; Hruby, M. Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent 19F MRI Tracer. Nanomaterials 2020, 10, 2231. https://doi.org/10.3390/nano10112231

AMA Style

Babuka D, Kolouchova K, Groborz O, Tosner Z, Zhigunov A, Stepanek P, Hruby M. Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent 19F MRI Tracer. Nanomaterials. 2020; 10(11):2231. https://doi.org/10.3390/nano10112231

Chicago/Turabian Style

Babuka, David, Kristyna Kolouchova, Ondrej Groborz, Zdenek Tosner, Alexander Zhigunov, Petr Stepanek, and Martin Hruby. 2020. "Internal Structure of Thermoresponsive Physically Crosslinked Nanogel of Poly[N-(2-hydroxypropyl)methacrylamide]-Block-Poly[N-(2,2-difluoroethyl)acrylamide], Prominent 19F MRI Tracer" Nanomaterials 10, no. 11: 2231. https://doi.org/10.3390/nano10112231

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop