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J. Funct. Biomater. 2015, 6(3), 667-686;

Compositional and in Vitro Evaluation of Nonwoven Type I Collagen/Poly-dl-lactic Acid Scaffolds for Bone Regeneration

Biomaterials and Tissue Engineering Research Group, School of Dentistry, University of Leeds, Leeds LS2 9LU, UK
Nonwovens Research Group, School of Design, University of Leeds, Leeds LS2 9JT, UK
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China
Author to whom correspondence should be addressed.
Academic Editor: Francesco Puoci
Received: 30 May 2015 / Revised: 20 July 2015 / Accepted: 30 July 2015 / Published: 5 August 2015
(This article belongs to the Special Issue Medical Textiles)
Full-Text   |   PDF [2989 KB, uploaded 5 August 2015]   |  


Poly-dl-lactic acid (PDLLA) was blended with type I collagen to attempt to overcome the instantaneous gelation of electrospun collagen scaffolds in biological environments. Scaffolds based on blends of type I collagen and PDLLA were investigated for material stability in cell culture conditions (37 °C; 5% CO2) in which post-electrospinning glutaraldehyde crosslinking was also applied. The resulting wet-stable webs were cultured with bone marrow stromal cells (HBMSC) for five weeks. Scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), Fourier transform infra-red spectroscopy (FTIR) and biochemical assays were used to characterise the scaffolds and the consequent cell-scaffold constructs. To investigate any electrospinning-induced denaturation of collagen, identical PDLLA/collagen and PDLLA/gelatine blends were electrospun and their potential to promote osteogenic differentiation investigated. PDLLA/collagen blends with w/w ratios of 40/60, 60/40 and 80/20 resulted in satisfactory wet stabilities in a humid environment, although chemical crosslinking was essential to ensure long term material cell culture. Scaffolds of PDLLA/collagen at a 60:40 weight ratio provided the greatest stability over a five-week culture period. The PDLLA/collagen scaffolds promoted greater cell proliferation and osteogenic differentiation compared to HMBSCs seeded on the corresponding PDLLA/gelatine scaffolds, suggesting that any electrospinning-induced collagen denaturation did not affect material biofunctionality within 5 weeks in vitro. View Full-Text
Keywords: electrospinning; collagen; poly-dl-lactic acid; crosslinking; nonwoven electrospinning; collagen; poly-dl-lactic acid; crosslinking; nonwoven

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Qiao, X.; Russell, S.J.; Yang, X.; Tronci, G.; Wood, D.J. Compositional and in Vitro Evaluation of Nonwoven Type I Collagen/Poly-dl-lactic Acid Scaffolds for Bone Regeneration. J. Funct. Biomater. 2015, 6, 667-686.

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