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Article

HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation

by
Jorge López-García
1,
Marián Lehocký
1,*,
Petr Humpolíček
1,2 and
Petr Sáha
1
1
Centre of Polymer Systems, Polymer Centre, Tomas Bata University in Zlin, T.G.Masaryk Sq. 5555, 76005 Zlin, Czech Republic
2
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.Masaryk Sq. 275, 76272 Zlin, Czech Republic
*
Author to whom correspondence should be addressed.
J. Funct. Biomater. 2014, 5(2), 43-57; https://doi.org/10.3390/jfb5020043
Submission received: 9 December 2013 / Revised: 7 March 2014 / Accepted: 2 April 2014 / Published: 8 May 2014
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Abstract

The effective and widely tested biocides: Benzalkonium chloride, bronopol, chitosan, chlorhexidine and irgasan were added in different concentrations to atelocollagen matrices. In order to assess how these antibacterial agents influence keratinocytes cell growth, cell viability and proliferation were determined by using MTT assay. Acquired data indicated a low toxicity by employing any of these chemical substances. Furthermore, cell viability and proliferation were comparatively similar to the samples where there were no biocides. It means that regardless of the agent, collagen-cell-attachment properties are not drastically affected by the incorporation of those biocides into the substrate. Therefore, these findings suggest that these atelocollagen substrates enhanced by the addition of one or more of these agents may render effectiveness against bacterial stains and biofilm formation, being the samples referred to herein as “antimicrobial substrates” a promising view in the design of novel antimicrobial biomaterials potentially suitable for tissue engineering applications.
Keywords: atelocollagen; antibacterial surface; cytotoxicity; cell proliferation; MTT assay atelocollagen; antibacterial surface; cytotoxicity; cell proliferation; MTT assay

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MDPI and ACS Style

López-García, J.; Lehocký, M.; Humpolíček, P.; Sáha, P. HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation. J. Funct. Biomater. 2014, 5, 43-57. https://doi.org/10.3390/jfb5020043

AMA Style

López-García J, Lehocký M, Humpolíček P, Sáha P. HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation. Journal of Functional Biomaterials. 2014; 5(2):43-57. https://doi.org/10.3390/jfb5020043

Chicago/Turabian Style

López-García, Jorge, Marián Lehocký, Petr Humpolíček, and Petr Sáha. 2014. "HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation" Journal of Functional Biomaterials 5, no. 2: 43-57. https://doi.org/10.3390/jfb5020043

APA Style

López-García, J., Lehocký, M., Humpolíček, P., & Sáha, P. (2014). HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation. Journal of Functional Biomaterials, 5(2), 43-57. https://doi.org/10.3390/jfb5020043

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