Cell Attachment to Hydrogel-Electrospun Fiber Mat Composite Materials
Abstract
:1. Introduction
2. Results and Discussion
Material 1 | Topography | Surface Composition |
---|---|---|
PEG G | Gel | PEG |
PEGPCL G | Gel | PEGPCL |
PCL EFM | Fibers | PCL |
PCL/PEGPCL 2 EFM | Fibers | PEGPCL |
PEGPCL G-PCL EFM | Composite | PCL |
PEGPCL G-PCL/PEGPCL2 EFM | Composite | PEGPCL |
2.1. Characterization of Electrospun Fiber Mats
EFM | Water contact angle (°) | Average fiber width ω (µm) | Mean pore size (µm) | Porosity ε (%) | Available surface fraction (1 − Φ) |
---|---|---|---|---|---|
PCL | 135.2 ± 5.4 | 1.726 ± 0.102 | 1.517 ± 0.203 | 82 ± 2 | ~0.79 |
PCL/PEGPCL | ~0 | 1.512 ± 0.141 | 1.557 ± 0.229 | 83 ± 2 | ~0.73 |
2.2. SK-N-SH Neuroblastoma Cell Attachment and Proliferation
2.3. Rat Cortical Cell Attachment to Composite Materials
3. Experimental Section
3.1. Electrospun Fiber Mat Fabrication
3.1.1. PCL Mat Fabrication
3.1.2. PCL/PEGPCL Core/Shell Mat Fabrication
3.2. Electrospun Fiber Mat Characterization
3.3. Synthesis of Diacryl-PEGPCL Copolymer
3.4. Formation of Hydrogels and Hydrogel-Electrospun Fiber Mat Composite Materials
3.4.1. PEG and PEGPCL Hydrogels
3.4.2. Composite Materials
3.5. SK-N-SH Cell Culture
3.6. SK-N-SH Cell Fluorescent Imaging and the MTT Proliferation Assay
3.7. Rat Cortical Neuron Culture
3.8. Cell Fixation and Scaffold SEM Imaging
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Han, N.; Johnson, J.K.; Bradley, P.A.; Parikh, K.S.; Lannutti, J.J.; Winter, J.O. Cell Attachment to Hydrogel-Electrospun Fiber Mat Composite Materials. J. Funct. Biomater. 2012, 3, 497-513. https://doi.org/10.3390/jfb3030497
Han N, Johnson JK, Bradley PA, Parikh KS, Lannutti JJ, Winter JO. Cell Attachment to Hydrogel-Electrospun Fiber Mat Composite Materials. Journal of Functional Biomaterials. 2012; 3(3):497-513. https://doi.org/10.3390/jfb3030497
Chicago/Turabian StyleHan, Ning, Jed K. Johnson, Patrick A. Bradley, Kunal S. Parikh, John J. Lannutti, and Jessica O. Winter. 2012. "Cell Attachment to Hydrogel-Electrospun Fiber Mat Composite Materials" Journal of Functional Biomaterials 3, no. 3: 497-513. https://doi.org/10.3390/jfb3030497